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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/77184
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dc.contributor.authorLi, Hongbing-
dc.contributor.authorRuano, María José-
dc.contributor.authorSánchez-González, Pablo-
dc.contributor.authorVillalobo, Antonio-
dc.date.accessioned2013-05-30T09:41:33Z-
dc.date.available2013-05-30T09:41:33Z-
dc.date.issued2012-
dc.identifierdoi: 10.1074/jbc.M111.317529-
dc.identifierissn: 0021-9258-
dc.identifiere-issn: 1083-351X-
dc.identifier.citationJournal of Biological Chemistry 287(5): 3273-3281 (2012)-
dc.identifier.urihttp://hdl.handle.net/10261/77184-
dc.descriptionet al.-
dc.description.abstractCalmodulin (CaM) is the major component of calcium signaling pathways mediating the action of various effectors. Transient increases in the intracellular calcium level triggered by a variety of stimuli lead to the formation of Ca2+/CaM complexes, which interact with and activate target proteins. In the present study the role of Ca2+/CaM in the regulation of the ligand-dependent activation of the epidermal growth factor receptor (EGFR) has been examined in living cells. We show that addition of different cell permeable CaM antagonists to cultured cells or loading cells with a Ca2+ chelator inhibited ligand-dependent EGFR auto(trans)phosphorylation. This occurred also in the presence of inhibitors of protein kinase C, CaM-dependent protein kinase II and calcineurin, which are known Ca2+- and/or Ca2+/CaM-dependent EGFR regulators, pointing to a direct effect of Ca2+/CaM on the receptor. Furthermore, we demonstrate that down-regulation of CaM in conditional CaM knock out cells stably transfected with the human EGFR decreased its ligand-dependent phosphorylation. Substitution of six basic amino acid residues within the CaM-binding domain (CaM-BD) of the EGFR by alanine resulted in a decreased phosphorylation of the receptor and of its downstream substrate phospholipase Cγ1. These results support the hypothesis that Ca2+/CaM regulates the EGFR activity by directly interacting with the CaM-BD of the receptor located at its cytosolic juxtamembrane region.-
dc.description.sponsorshipThis work was financed by grants (to A. V.) from the Ministerio de Ciencia e Innovación (SAF2008-00986 and SAF2011-23494) and the European Commission (contract ITN 289033); Grant from the Danish Research Council (to M. W. B.), the Lundbeck Foundation, AP Møller Fonden, Dagmar Marshall Fonden, Willumsen Fonden, Danielsen Fonden, and Wedell Wedellsborg Fonden.-
dc.language.isoeng-
dc.publisherAmerican Society for Biochemistry and Molecular Biology-
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/289033-
dc.rightsclosedAccess-
dc.titleRegulation of the ligand-dependent activation of the epidermal growth factor receptor by calmodulin-
dc.typeartículo-
dc.identifier.doi10.1074/jbc.M111.317529-
dc.date.updated2013-05-30T09:41:33Z-
dc.description.versionPeer Reviewed-
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderEuropean Commission-
dc.contributor.funderDanish Council for Independent Research-
dc.contributor.funderLundbeck Foundation-
dc.contributor.funderVillum Fonden-
dc.relation.csicSí-
dc.relation.csicSí-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004837es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003554es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100008398es_ES
dc.identifier.pmid22157759-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
item.languageiso639-1en-
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