English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/7717
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Enhancement of DNA, cDNA synthesis and fidelity at high temperatures by a dimeric single-stranded DNA-binding protein

AutorPerales, Celia ; Cava, Felipe ; Meijer, Wilfried J. J. ; Berenguer, José
Palabras claveThermus thermophilus
Pyrococcus furiosus
Fecha de publicación2003
EditorOxford University Press
CitaciónNucleic Acids Research, 2003, Vol. 31, No. 22 6473-6480
ResumenBacterial single-stranded DNA-binding proteins (SSBs) are required for DNA replication and repair. We have over-expressed and purified the native form and two His-tagged fusions of the SSB from Thermus thermophilus (TthSSB). The three proteins were found as dimers in solution. They bound in vitro to single-stranded DNA specifically over a temperature range of 4–80°C, and the wild-type protein could withstand incubation at 94°C for 2 min. Addition of TthSSB to PCR halved the elongation time required for the DNA polymerases of T.thermophilus (Tth) and Pyrococcus furiosus (Pfu) to synthesise DNA fragments in PCRs. The presence of TthSSB increased the fidelity of the proof- reading-free DNA polymerase of T.thermophilus. TthSSB was also able to bind single-stranded RNA, allowing a dramatic enhancement of the reverse transcription activity of its cognate Tth DNA polymerase during cDNA synthesis.
Versión del editorhttp://dx.doi.org/10.1093/nar/gkg865
URIhttp://hdl.handle.net/10261/7717
DOI10.1093/nar/gkg865
ISSN0305-1048 (print)
1362-4962 (online)
Aparece en las colecciones: (CBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
JBerenguer_NAR_6473.pdf174,03 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.