English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/76761
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Effects of polyethylene and α-alumina particles on IL-6 expression and secretion in primary cultures of human osteoblastic cells

AuthorsRodrigo, A. M.; Martínez, M. E.; Saldaña, L.; Vallés, G.; Martínez, P.; González-Carrasco, José Luis CSIC ORCID ; Cordero, J.; Munuera, L.
Issue Date2002
CitationBiomaterials 23: 901-908 (2002)
AbstractThe effect of two biomaterials, polyethylene and α-alumina, on interleukin-6 (IL-6) secretion and expression has been studied in human osteoblasts in primary culture. Human osteoblastic cells were derived from fresh trabecular bone explants removed during total knee arthroplasty. On reaching confluence, cells were subcultured in 6 well plates; the resulting subcultures were incubated until confluence and polyethylene or α-alumina particles were added to some while the rest were left as controls. The IL-6 mRNA levels were assessed by reverse transcription (RT) followed by polymerase chain reaction (PCR). IL-6 secretion was measured in the conditioned medium. The IL-6 expression was higher in the presence of both biomaterials. Maximum expression occurred in response to a dose of 50mg particles/well with both biomaterials and was greater after polyethylene particle addition than after α-alumina particle addition at this dose. The maximum IL-6 secretion elicited by α-alumina was produced at 10mg particles/well while maximum response with polyethylene required 50mg/well. At a dose of 10mg/well, α-alumina particles induced more secretion than 10mg of polyethylene particles. Nevertheless, at a dose of 50mg/well maximum secretion was produced with polyethylene particles. In conclusion and in our experimental conditions, polyethylene as well as α-alumina increased both the expression and the secretion of IL-6 in human osteoblastic cells in primary culture and stimulation from polyethylene appears stronger than that from α-alumina at the same dose. © 2001 Elsevier Science Ltd. All rights reserved.
Identifiersdoi: 10.1016/S0142-9612(01)00200-9
issn: 0142-9612
Appears in Collections:(CENIM) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.