Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/7629
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dc.contributor.authorOrtega, Richard-
dc.contributor.authorCarmona, Asunción-
dc.date.accessioned2008-10-10T17:03:53Z-
dc.date.available2008-10-10T17:03:53Z-
dc.date.issued2007-09-26-
dc.identifier.citationPLoS ONE 2(9): e925 (2007)en_US
dc.identifier.urihttp://hdl.handle.net/10261/7629-
dc.description6 pages.-- PMCID: PMC1976597.-- et al.en_US
dc.description.abstractAltered homeostasis of metal ions is suspected to play a critical role in neurodegeneration. However, the lack of analytical technique with sufficient spatial resolution prevents the investigation of metals distribution in neurons. An original experimental setup was developed to perform chemical element imaging with a 90 nm spatial resolution using synchrotron-based X-ray fluorescence. This unique spatial resolution, combined to a high brightness, enables chemical element imaging in subcellular compartments. We investigated the distribution of iron in dopamine producing neurons because iron-dopamine compounds are suspected to be formed but have yet never been observed in cells. The study shows that iron accumulates into dopamine neurovesicles. In addition, the inhibition of dopamine synthesis results in a decreased vesicular storage of iron. These results indicate a new physiological role for dopamine in iron buffering within normal dopamine producing cells. This system could be at fault in Parkinson’s disease which is characterized by an increased level of iron in the substancia nigra pars compacta and an impaired storage of dopamine due to the disruption of vesicular trafficking. The re-distribution of highly reactive dopamine-iron complexes outside neurovesicles would result in an enhanced death of dopaminergic neurons.en_US
dc.description.sponsorshipThe development of these experiments was supported by the European Synchrotron Radiation Facility, and the European program of integrated action ‘Picasso’.en_US
dc.format.extent357345 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofPublisher’s version-
dc.rightsopenAccessen_US
dc.subjectNeuronsen_US
dc.subjectMetals distributionen_US
dc.subjectAnalytical techniquesen_US
dc.subjectSynchrotron-baseden_US
dc.subjectDopamine-producing-neuronsen_US
dc.subjectIronen_US
dc.subjectDopamine neurovesiclesen_US
dc.subjectNeurodegenerationen_US
dc.titleIron storage within dopamine neurovesicles revealed by chemical nano-imagingen_US
dc.typeartículoen_US
dc.identifier.doi10.1371/journal.pone.0000925-
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://dx.doi.org/10.1371/journal.pone.0000925en_US
dc.identifier.e-issn1932-6203-
dc.identifier.pmid17895967-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.fulltextWith Fulltext-
item.grantfulltextopen-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
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