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Title

Iron storage within dopamine neurovesicles revealed by chemical nano-imaging

AuthorsOrtega, Richard; Carmona, Asunción
KeywordsNeurons
Metals distribution
Analytical techniques
Synchrotron-based
Dopamine-producing-neurons
Iron
Dopamine neurovesicles
Neurodegeneration
Issue Date26-Sep-2007
PublisherPublic Library of Science
CitationPLoS ONE 2(9): e925 (2007)
AbstractAltered homeostasis of metal ions is suspected to play a critical role in neurodegeneration. However, the lack of analytical technique with sufficient spatial resolution prevents the investigation of metals distribution in neurons. An original experimental setup was developed to perform chemical element imaging with a 90 nm spatial resolution using synchrotron-based X-ray fluorescence. This unique spatial resolution, combined to a high brightness, enables chemical element imaging in subcellular compartments. We investigated the distribution of iron in dopamine producing neurons because iron-dopamine compounds are suspected to be formed but have yet never been observed in cells. The study shows that iron accumulates into dopamine neurovesicles. In addition, the inhibition of dopamine synthesis results in a decreased vesicular storage of iron. These results indicate a new physiological role for dopamine in iron buffering within normal dopamine producing cells. This system could be at fault in Parkinson’s disease which is characterized by an increased level of iron in the substancia nigra pars compacta and an impaired storage of dopamine due to the disruption of vesicular trafficking. The re-distribution of highly reactive dopamine-iron complexes outside neurovesicles would result in an enhanced death of dopaminergic neurons.
Description6 pages.-- PMCID: PMC1976597.-- et al.
Publisher version (URL)http://dx.doi.org/10.1371/journal.pone.0000925
URIhttp://hdl.handle.net/10261/7629
DOI10.1371/journal.pone.0000925
E-ISSN1932-6203
Appears in Collections:(CNA) Artículos
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