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Title

A critical appraisal of quantitative studies of protein degradation in the framework of cellular proteostasis

AuthorsÁlvarez-Castelao, Beatriz; Ruiz-Rivas, Carmen; Castaño, José G.
Issue Date2012
PublisherHindawi Publishing Corporation
CitationBiochemistry Research International: 823597 (2012)
AbstractProtein homeostasis, proteostasis, is essential to understand cell function. Protein degradation is a crucial component of the proteostatic mechanisms of the cell. Experiments on protein degradation are nowadays present in many investigations in the field of molecular and cell biology. In the present paper, we focus on the different experimental approaches to study protein degradation and present a critical appraisal of the results derived from steady-state and kinetic experiments using detection of unlabelled and labelled protein methodologies with a proteostatic perspective. This perspective allows pinpointing the limitations in interpretation of results and the need of further experiments and/or controls to establish definitive evidence for the role of protein degradation in the proteostasis of a given protein or the entire proteome. We also provide a spreadsheet for simple calculations of mRNA and protein decays for mimicking different experimental conditions and a checklist for the analysis of experiments dealing with protein degradation studies that may be useful for researchers interested in the area of protein turnover. © 2012 Beatriz Alvarez-Castelao et al.
Publisher version (URL)http://dx.doi.org/10.1155/2012/823597
URIhttp://hdl.handle.net/10261/76186
DOI10.1155/2012/823597
Identifiersdoi: 10.1155/2012/823597
issn: 2090-2247
e-issn: 2090-2255
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