English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/7618
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 25 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título : Excitation energy transfer in chlorosomes of Chlorobium phaeobacteroides strain CL1401: the role of carotenoids
Autor : Psencik, Jakub; Ma, Ying-Zhong; Arellano, Juan B. ; García-Gil, L. Jesús; Holzwarth, Alfred R.; Gillbro, Tomas
Palabras clave : bacteriochlorophyll
carotenoid
chlorosome
Chlorobiaceae
energy transfer
femtosecond spectroscopy
fluorescence excitation
green sulfur bacteria
single-photon timing
Fecha de publicación : nov-2002
Editor: Springer
Citación : Photosynthesis Research (2002) 71:5-18
Resumen: The role of carotenoids in chlorosomes of the green sulfur bacterium Chlorobium phaeobacteroides, containing bacteriochlorophyll (BChl) e and the carotenoid (Car) isorenieratene as main pigments, was studied by steady-state fluorescence excitation, picosecond single-photon timing and femtosecond transient absorption (TA) spectroscopy. In order to obtain information about energy transfer from Cars in this photosynthetic light-harvesting antenna with high spectral overlap between Cars and BChls, Car-depleted chlorosomes, obtained by inhibition of Car biosynthesis by 2-hydroxybiphenyl, were employed in a comparative study with control chlorosomes. Excitation spectra measured at room temperature give an efficiency of 60-70% for the excitation energy transfer from Cars to BChls in control chlorosomes. Femtosecond TA measurements enabled an identification of the excited state absorption band of Cars and the lifetime of their S-1 state was determined to be similar to10 ps. Based on this lifetime, we concluded that the involvement of this state in energy transfer is unlikely. Furthermore, evidence was obtained for the presence of an ultrafast (<100 fs) energy transfer process from the S-2 state of Cars to BChls in control chlorosomes. Using two time-resolved techniques, we further found that the absence of Cars leads to overall slower decay kinetics probed within the Qy band of BChl e aggregates, and that two time constants are generally required to describe energy transfer from aggregated BChl e to baseplate BChl a.
Versión del editor: http://www.springerlink.com/content/nwvtdvnlu1n0atpv/?p=b2b8dcf01d9242358e430be8eb155c85&pi=1
URI : http://hdl.handle.net/10261/7618
DOI: 10.1023/A:1014943312031
Aparece en las colecciones: (IRNASA) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Psencik et al. 2002.pdf379,7 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.