Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/75380
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Tormo-Más, María Ángeles | - |
dc.contributor.author | Donderis, Jorge | - |
dc.contributor.author | García-Caballer, María | - |
dc.contributor.author | Alt, Aaron | - |
dc.contributor.author | Mir-Sanchis, Ignacio | - |
dc.contributor.author | Marina, Alberto | - |
dc.contributor.author | Penadés, José R. | - |
dc.date.accessioned | 2013-04-30T10:41:03Z | - |
dc.date.available | 2013-04-30T10:41:03Z | - |
dc.date.issued | 2013-01-17 | - |
dc.identifier.citation | Molecular Cell 49(5):947-58 (2013) | es_ES |
dc.identifier.issn | 1097-2765 | - |
dc.identifier.uri | http://hdl.handle.net/10261/75380 | - |
dc.description | 12 páginas, 7 figuras, 1 tabla. Se incluye post-print y material suplementario en fichero. | es_ES |
dc.description.abstract | dUTPases (Duts) have emerged as promising regulatory molecules controlling relevant cellular processes. However, the mechanism underlying this regulatory function remains enigmatic. Using staphylococcal pathogenicity island (SaPI) repression as a model, we report here that phage Duts induce the transfer of SaPI-encoded virulence factors by switching between active (dUTP-bound) and inactive (apo state) conformations, a conversion catalyzed by their intrinsic dUTPase activity. Crystallographic and mutagenic analyses demonstrate that binding to dUTP reorders the C-terminal motif V of the phage-encoded Duts, rendering these proteins into the active conformation required for SaPI derepression. By contrast, the conversion to the apo state conformation by hydrolysis of the bound dUTP generates a protein that is unable to induce the SaPI cycle. Because none of the requirements involving Duts in SaPI transfer are exclusive to the phage-encoded proteins, we propose that Duts are widespread cellular regulators acting in a manner analogous to the eukaryotic G proteins. | es_ES |
dc.description.sponsorship | This work was supported by grants BIO2011-30503-C02-01 and Eranet-pathogenomics PIM2010EPA-00606 to J.R.P and BIO2010-15424 to A.M. from the Ministerio de Ciencia e Innovación (MICINN), grants from the Cardenal Herrera-CEU University (Copernicus-Santander program) to J.R.P., grants ACOMP/2012/044 from Generalitat Valenciana to A.M., and grants Consolider-Ingenio CSD2009-00006 and INIA-DR08-0093 to M.A.T.-M. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.publisher | Cell Press | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Staphylococcus aureus | es_ES |
dc.subject | Phage | es_ES |
dc.subject | Pathogenicity island | es_ES |
dc.subject | SaPI induction | es_ES |
dc.subject | Gene transfer | es_ES |
dc.subject | Moonlighting proteins | es_ES |
dc.subject | dUTPase | es_ES |
dc.subject | dUTP | es_ES |
dc.subject | G protein | es_ES |
dc.subject | P-loop | es_ES |
dc.title | Phage dUTPases control transfer of virulence genes by a proto-oncogenic G protein-like mechanism | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1016/j.molcel.2012.12.013 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.molcel.2012.12.013 | es_ES |
dc.identifier.e-issn | 1097-4164 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.languageiso639-1 | en | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
Aparece en las colecciones: | (IBV) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
2013 MOLECULAR-CELL.pdf | 12,99 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
47
checked on 03-abr-2024
WEB OF SCIENCETM
Citations
45
checked on 24-feb-2024
Page view(s)
382
checked on 22-abr-2024
Download(s)
278
checked on 22-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.