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dc.contributor.authorCampo, Javier del-
dc.contributor.authorNot, Fabrice-
dc.contributor.authorForn, Irene-
dc.contributor.authorSieracki, Michael E.-
dc.contributor.authorMassana, Ramon-
dc.identifier.citationISME Journal - International Society for Microbial Ecology 7: 351-358 (2012)es_ES
dc.description8 páginas, 3 figuras, 2 tables.es_ES
dc.description.abstractProtists (unicellular eukaryotes) arguably account for most eukaryotic diversity and are central players of the biosphere. Known protist diversity and biology is largely based on cultured strains. Yet, environmental molecular surveys have unveiled entirely novel lineages that, as their prokaryotic counterparts, are essentially uncultured. Culture bias is an important drawback for any microbe-related science and is particularly severe for heterotrophic protists, which depend on organic food sources for growth. Here, we show how ecologically significant bacterivorous protists have been brought into culture by mimicking in situ conditions. Single cells sorted by serial dilution or flow cytometry were inoculated into seawater amended with natural bacterial assemblage at nearly in situ abundances. Strains belonging to lineages only known so far from environmental sequencing were isolated. Among them, Minorisa minuta gen. nov. sp. nov. forms a novel branch within Rhizaria, holding a key evolutionary position, and with an average size of 1.4 μm represents one of the smallest bacterial grazers known to date. It has a worldwide planktonic distribution and can account for 5% of heterotrophic protists communities in coastal waters. Physiological features of this strain can partly explain its success in the environment. Culturing ecologically relevant but elusive protists provide invaluable material for ecophysiology, genomics, ecosystem modeling and evolutionary issues.es_ES
dc.description.sponsorshipThis study was supported by projects MICROVIS (CTM2007–62140/MAR, MEC), FLAME (CGL2010– 16304, MICINN) and BioMarKs (2008–6530, ERA-net Biodiversa, EU). JdC was funded by the I3P program (I3PPRE-06–00676, CSIC) and FN by the Marie-Curie fellowship ESUMAST (MEIF-CT-2005–025000). We thank Marco Álvarez for help in TSA-FISH, Vanessa Balagué for help in molecular analysis, José Manuel Fortuño for help in SEM and Nicole Poulton for help in flow cytometry.es_ES
dc.publisherInternational Society for Microbial Ecologyes_ES
dc.subjectSingle celles_ES
dc.subjectHeterotrophic flagellateses_ES
dc.titleTaming the smallest predators of the oceanses_ES
dc.description.peerreviewedPeer reviewedes_ES
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