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dc.contributor.authorLlull, Daniel-
dc.contributor.authorMuñoz, Rosario-
dc.contributor.authorGarcía, Ernesto-
dc.identifierissn: 0022-1007-
dc.identifiere-issn: 1540-9538-
dc.identifier.citationJournal of Experimental Medicine 190(2): 241-251 (1999)-
dc.description.abstractThe molecular aspects of the type 37 pneumococcal capsular biosynthesis, a homopolysaccharide composed of sophorosyl units (β-D-Glc-(1→2)-β-D-Glc) linked by β-1,3 bonds, have been studied. Remarkably, the biosynthesis of the type 37 capsule is driven by a single gene (tts) located far apart from the cap locus responsible for capsular formation in all of the types characterized to date in Streptococcus pneumoniae. However, a cap37 locus virtually identical to the cap33f cluster has been found in type 37 strains, although some of its genes are inactivated by mutations. The tts gene has been sequenced and its transcription start point determined. Tts shows sequence motifs characteristic of cellulose synthases and other β- glycosyltransferases. Insertion of the tts gene into the pneumococcal DNA causes a noticeable genome reorganization in such a way that genes normally separated by more than 350 kb in the chromosome are located together in clinical isolates of type 37. Encapsulated pneumococcal strains belonging to 10 different serotypes (or serogroups) transformed with tts synthesized type 37 polysaccharide, leading to the formation of strains that display the binary type of capsule. Type 37 pneumococcus constitutes the first case of a natural, genetically binary strain and represents a novel alternative to the mechanisms of intertype transformation.-
dc.description.sponsorshipWe thank P. García, J.L. García, and E. Díaz for their critical reading of the manuscript. R. Muñoz was the recipient of a Contrato Temporal de Investigadores from the CSIC, and D. Llull was the recipient of a fellowship from Programa Sectorial de Formación de Profesorado Universitario y Personal Investigador (Ministerio de Educación y Cultura). This study was supported by grant PB96-0809 from the Dirección General de Investigación Científica y Técnica. Preliminary sequence data were obtained from The Institute for Genomic Research (TIGR) through the website at http://www.tigr.org. Sequencing of the S. pneumoniae genome was accomplished with support from TIGR, the National Institute of Allergy and Infectious Diseases (NIAID), and the Merck Genome Research Institute (MGRI).-
dc.publisherRockefeller University Press-
dc.relation.isversionofPublisher's version-
dc.titleA single gene (tts) located outside the cap locus directs the formation of Streptococcus pneumoniae type 37 capsular polysaccharide: Type 37 pneumococci are natural, genetically binary strains-
dc.description.versionPeer Reviewed-
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