English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/74319
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Genetic diversity and relatedness of sweet cherry (prunus avium L.) cultivars based on single nucleotide polymorphic markers.

AuthorsFernández i Marti, Ángel; Athanson, Blessing; Koepke, Tyson; Font i Forcada, Carolina ; Dhingra, Amit; Oraguzie, Nnadozie
KeywordsPrunus avium L.
3 UTR sequencing
high resolution melting analysis
molecular markers rentageverification
genetic parameters
parentage verification
Issue Date25-Jun-2012
PublisherFrontiers Media
CitationFernández i Martí A, Athanson B, Koepke T, Font i Forcada C, Dhingra A, Oraguzie N. Genetic diversity and relatedness of sweet cherry (prunus avium L.) cultivars based on single nucleotide polymorphic markers. Frontiers in Plan Science 3 (116) (2012)
AbstractMost previous studies on genetic fingerprinting and cultivar relatedness in sweet cherry were based on isoenzyme, RAPD, and simple sequence repeat (SSR) markers. This study was carried out to assess the utility of single nucleotide polymorphism (SNP) markers generated from 3' untranslated regions (UTR) for genetic fingerprinting in sweet cherry. A total of 114 sweet cherry germplasm representing advanced selections, commercial cultivars, and old cultivars imported from different parts of the world were screened with seven SSR markers developed from other Prunus species and with 40 SNPs obtained from 3' UTR sequences of Rainier and Bing sweet cherry cultivars. Both types of marker study had 99 accessions in common. The SSR data was used to validate the SNP results. Results showed that the average number of alleles per locus, mean observed heterozygosity, expected heterozygosity, and polymorphic information content values were higher in SSRs than in SNPs although both set of markers were similar in their grouping of the sweet cherry accessions as shown in the dendrogram. SNPs were able to distinguish sport mutants from their wild type germplasm. For example, "Stella" was separated from "Compact Stella." This demonstrates the greater power of SNPs for discriminating mutants from their original parents than SSRs. In addition, SNP markers confirmed parentage and also determined relationships of the accessions in a manner consistent with their pedigree relationships. We would recommend the use of 3' UTR SNPs for genetic fingerprinting, parentage verification, gene mapping, and study of genetic diversity in sweet cherry.
Description13 Pags., 5 Tabls., 2 Figs.
Publisher version (URL)http://dx.doi.org/10.3389/fpls.2012.00116
URIhttp://hdl.handle.net/10261/74319
DOI10.3389/fpls.2012.00116
E-ISSN1664-462X
Appears in Collections:(EEAD) Artículos
Files in This Item:
File Description SizeFormat 
FontC_FrontPlantSci_2012.pdf1,25 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.