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Fluorescent protein vectors for promoter analysis in lactic acid bacteria and Escherichia coli

AuthorsGarcía-Cayuela, Tomás ; Gómez de Cadiñanos, Luz P.; Mohedano Bonillo, Mari Luz ; Fernández de Palencia, P. ; Boden, Daniel; Wells, Jerry; Peláez, Carmen ; López, Paloma ; Requena, Teresa
Issue Date2012
CitationApplied Microbiology and Biotechnology 96(1): 171-181 (2012)
AbstractFluorescent reporter genes are valuable tools for real-time monitoring of gene expression in living cells. In this study we describe the construction of novel promoter-probe vectors containing a synthetic mCherry fluorescent protein gene, codon-optimized for lactic acid bacteria, divergently linked, or not, to a gene encoding the S65T and F64L variant of the green fluorescent protein. The utility of the transcriptional fusion vectors was demonstrated by the cloning of a single or two divergent promoter regions and by the quantitative evaluation of fluorescence during growth of Lactococcus lactis, Enterococcus faecalis, and Escherichia coli.
Description29 p.- 4 tab.-2 fig.
Publisher version (URL)http://dx.doi.org/10.1007/s00253-012-4087-z
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