English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/73567
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Changes induced by fe deficiency and fe resupply in the root protein profile of a peach-almond hybrid rootstock

AuthorsRodríguez-Celma, Jorge CSIC ORCID; Lattanzio, Giuseppe CSIC ORCID CVN; Jiménez Tarodo, Sergio CSIC; Briat, Jean François; Abadía Bayona, Javier CSIC ORCID ; Abadía Bayona, Anunciación CSIC ORCID ; Gogorcena Aoiz, Yolanda CSIC ORCID ; López-Millán, Ana Flor CSIC
two-dimensional gel electrophoresis
Issue DateMar-2013
PublisherAmerican Chemical Society
CitationRodríguez-Celma J, Lattanzio G, Jiménez S, Briat JF, Abadía J, Abadía A, Gogorcena Y, López-Millán AF. Changes induced by fe deficiency and fe resupply in the root protein profile of a peach-almond hybrid rootstock. Journal of Proteome Research 12 (3): 1162-1172 (2013)
AbstractThe changes in the root extract protein profile of the Prunus hybrid GF 677 rootstock (P. dulcis × P. persica) grown in hydroponics as affected by Fe deficiency and short-term (24 h) Fe resupply have been studied by 2-dimensional gel electrophoresis-based techniques. A total of 335 spots were consistently found in the gels. Iron deficiency caused above 2-fold increases or >50% decreases in the relative abundance in 10 and 6 spots, respectively, whereas one spot was only detected in Fe-deficient plants. Iron resupply to Fe-deficient plants caused increases and decreases in relative abundance in 15 and 16 spots, respectively, and one more spot was only detected in Fe-resupplied Fe-deficient plants. Ninety-five percent of the proteins changing in relative abundance were identified using nanoliquid chromatography-tandem mass spectrometry. Defense responses against oxidative and general stress accounted for 50% of the changes in Fe-deficient roots. Also, a slight induction of the glycolysis-fermentation pathways was observed in GF 677 roots with Fe deficiency. The root protein profile of 24 h Fe-resupplied plants was similar to that of Fe-deficient plants, indicating that the deactivation of Fe-deficiency metabolic responses is slow. Taken together, our results suggest that the high tolerance of GF 677 rootstock to Fe deficiency may be related to its ability to elicit a sound defense response against both general and oxidative stress.
Description11 Pags.
Publisher version (URL)http://dx.doi.org/10.1021/pr300763c
Appears in Collections:(EEAD) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.