English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/73019
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Functional recovery of troponin I in a Drosophila heldup mutant after a second site mutation

AuthorsPrado, Antonio; Canal, Inmaculada; Barbas, Julio A. CSIC; Molloy, J.; Ferrús, Alberto CSIC ORCID
Issue Date1995
PublisherAmerican Society for Cell Biology
CitationMolecular Biology of the Cell 6: 1433-1441 (1995)
AbstractTo identify proteins that interact in vivo with muscle components we have used a genetic approach based on the isolation of suppressors of mutant alleles of known muscle components. We have applied this system to the case of troponin I (TnI) in Drosophila and its mutant allele heldup2 (hdp2). This mutation causes an alanine to valine substitution at position 116 after a single nucleotide change in a constitutive exon. Among the isolated suppressors, one of them results from a second site mutation at the TnI gene itself. Muscles endowed with TnI mutated at both sites support nearly normal myofibrillar structure, perform notably well in wing heating and flight tests, and isolated muscle fibers produce active force. We show that the structural and functional recovery in this suppressor does not result from a change in the stoichiometric ratio of TnI isoforms. The second site suppression is due to a leucine to phenylalanine change within a heptameric leucine string motif adjacent to the actin binding domain of TnI. These data evidence a structural and functional role for the heptameric leucine string that is most noticeable, if not specific, in the indirect flight muscle.
Appears in Collections:(IC) Artículos
Files in This Item:
File Description SizeFormat 
Ferrus,1995,MolBiolCell.,6,1433-.pdf2,9 MBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.