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Title

Contribution of the different omega-3 fatty acid desaturase genes to the cold response in soybean

AuthorsRomán Fernández, Ángela ; Andreu Gargallo, Vanesa ; Hernández, M. Luisa ; Lagunas Castán, Beatriz ; Picorel Castaño, Rafael ; Martínez-Rivas, José Manuel ; Alfonso Lozano, Miguel
Keywordsdesaturase
FAD3
FAD7
FAD8
gene expression
cold
soybean
Issue DateAug-2012
PublisherOxford University Press
CitationRomán A, Andreu V, Hernández ML, Lagunas B, Picorel R, Martínez-Rivas JM, Alfonso M. Contribution of the different omega-3 fatty acid desaturase genes to the cold response in soybean. Journal of Experimental Botany 63 (13): 4973-4982 (2012)
AbstractThis study analysed the contribution of each omega-3 desaturase to the cold response in soybean. Exposure to cold temperatures (5 °C) did not result in great modifications of the linolenic acid content in leaf membrane lipids. However, an increase in the GmFAD3A transcripts was observed both in plant leaves and soybean cells whereas no changes in GmFAD3B or GmFAD3C expression levels were detected. This increase was reversible and accompanied by the accumulation of an mRNA encoding a truncated form of GmFAD3A (GmFAD3A-T), which originated from alternative splicing of GmFAD3A in response to cold. When the expression of plastidial omega-3 desaturases was analysed, a transient accumulation of GmFAD7-2 mRNA was detected upon cold exposure in mature soybean trifoliate leaves while GmFAD7-1 transcripts remained unchanged. No modification of the GmFAD8-1 and GmFAD8-2 transcripts was observed. The functionality of GmFAD3A, GmFAD3B, GmFAD3C and GmFAD3A-T was examined by heterologous expression in yeast. No activity was detected with GmFAD3A-T, consistent with the absence of one of the His boxes necessary for desaturase activity. The linolenic acid content of Sacharomyces cerevisiae cells overexpressing GmFAD3A or GmFAD3B was higher when the cultures were incubated at cooler temperatures, suggesting that reticular desaturases of the GmFAD3 family, and more specifically GmFAD3A, may play a role in the cold response, even in leaves. The data point to a regulatory mechanism of omega-3 fatty acid desaturases in soybean affecting specific isoforms in both the plastid and the endoplasmic reticulum to maintain appropriate levels of linolenic acid under low temperature conditions.
Description38 Pags., 1 Tabl., 7 Figs. The definitive version is available at: http://jxb.oxfordjournals.org/
Publisher version (URL)http://dx.doi.org/ 10.1093/jxb/ers174
URIhttp://hdl.handle.net/10261/72528
DOI10.1093/jxb/ers174
ISSN0022-0957
E-ISSN1460-2431
Appears in Collections:(EEAD) Artículos
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