Histochemical characteristics of diphosphate nucleoside consumption in cat and rat nervous system

Abstract

Consumption of diphosphate nucleosides was investigated by histochemical methods in rat and cat nervous system. The results show NDP-ase is found in histological sections from animals previously perfused with glutaraldehyde or formaldehyde. The histochemical reaction is increased in presence of Ca++, Mg++, Mn++ or imidazole, and inhibited by L-DOPA and noradrenaline in incubation medium. A comparative study of TPP-ase and NDP-ase activities is described to elucidate the identity of both enzymes.