English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/72491
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
DC FieldValueLanguage
dc.contributor.authorLópez-Sánchez, Carmen-
dc.contributor.authorBártulos Encinas, Óscar-
dc.contributor.authorMartínez-Campos, Enrique-
dc.contributor.authorGañán, Carlos-
dc.contributor.authorValenciano, Ana I.-
dc.contributor.authorGarcía-Martínez, Virginio-
dc.contributor.authorPablo, Flora de-
dc.contributor.authorHernández-Sánchez, Catalina-
dc.identifier.citationCardiovascular Research 88(1):111-120(2010)-
dc.description47 p.-5 fig.-6 supl.tab.-5 supl.fig.-
dc.description.abstractAimsTyrosine hydroxylase (TH) is the first and rate-limiting enzyme in catecholamine biosynthesis. Whereas the neuroendocrine roles of cathecolamines postnatally are well known, the presence and function of TH in organogenesis is unclear. The aim of this study was to define the expression of TH during cardiac development and to unravel the role it may play in heart formation. Methods and results: We studied TH expression in chick embryos by whole mount in situ hybridization and by quantitative reverse transcription-polymerase chain reaction and analysed TH activity by high-performance liquid chromatography. We used gain-and loss-of-function models to characterize the role of TH in early cardiogenesis. We found that TH expression was enriched in the cardiac field of gastrulating chick embryos. By stage 8, TH mRNA was restricted to the splanchnic mesoderm of both endocardial tubes and was subsequently expressed predominantly in the myocardial layer of the atrial segment. Overexpression of TH led to increased atrial myosin heavy chain (AMHC1) and T-box 5 gene (Tbx5) expression in the ventricular region and induced bradyarrhythmia. Similarly, addition of l-3,4-dihydroxyphenylalanine (l-DOPA) or dopamine induced ectopic expression of cardiac transcription factors (cNkx2.5, Tbx5) and AMHC1 as well as sarcomere formation. Conversely, blockage of dopamine biosynthesis and loss of TH activity decreased AMHC1 and Tbx5 expression, whereas exposure to retinoic acid (RA) induced TH expression in parallel to that of AMHC1 and Tbx5. Concordantly, inhibition of endogenous RA synthesis decreased TH expression as well as that of AMHC1 and Tbx5. Conclusion: TH is expressed in a dynamic pattern during the primitive heart tube formation. TH induces cardiac differentiation in vivo and it is a key regulator of the heart patterning, conferring atriogenic identity. © The Author 2010.-
dc.description.sponsorshipThis work was supported by the Spanish Ministry of Science and Innovation (MICINN) [BFU2007-61055 to C.H.-S., BFU2007-66350/BFI to C.L.-S.]; and the Junta de Extremadura [PRI07A005 to V.G.-M.]-
dc.publisherOxford University Press-
dc.subjectTyrosine hydroxylase-
dc.titleTyrosine hydroxylase is expressed during early heart development and is required for cardiac chamber formation-
dc.description.versionPeer Reviewed-
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
CVR-2010-9[1] combined submitted (REPOSITORIO).pdf1,3 MBAdobe PDFThumbnail
Show simple item record

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.