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Applications of Laser-Raman spectroscopy to the analysis of polynucleotides containing uridine residues

AuthorsEscobar, R.; Carmona, Pedro ; Molina, M.
Issue Date1991
CitationFresenius Journal of Analytical Chemistry 340: 213-216 (1991)
AbstractQualitative characterization of uridine C(2′)-endo-anti and C(3′)-endo-anti forms has been carried out for the first time using Laser-Raman spectroscopy. Spectra are presented for some crystals containing the uridine residue whose conformations are known from their X-ray crystallographic analyses. Crystalline uridine and uridine 5′-monophosphate as its lithium, sodium, potassium, barium and cadmium salts give Raman bands in the 600-700 cm-1 range that seem to reflect sensitively and regularly the conformation of the uridine residue and, therefore, are proposed as convenient indicators of the above structures. A method for the determination of the uridine C(2′)-endo-anti form in polyribonucleotides is also described. The usefulness of these characterizations in the conformational analysis of polyribonucleotides is shown. © 1991 Springer-Verlag.
Identifiersdoi: 10.1007/BF00321770
issn: 0937-0633
Appears in Collections:(CFMAC-IO) Artículos
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