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Título: | Lysophosphatidic acid inhibits Ca2+ signaling in response to epidermal growth factor receptor stimulation in human astrocytoma cells by a mechanism involving phospholipase Cγ and a G(αi) protein |
Autor: | Hernández, Marita CSIC ORCID CVN ; Barrero, María José; Sánchez Crespo, Mariano CSIC ORCID; Nieto, María Luisa CSIC ORCID | Fecha de publicación: | 2000 | Editor: | Wiley-Blackwell | Citación: | Journal of Neurochemistry 75(4): 1575-1582 (2000) | Resumen: | The effect of the lysophospholipid mediators lysophosphatidic acid (LPA) and sphingosine 1-phosphate and the polypeptide growth factor epidermal growth factor (EGF) on the human astrocytoma cell line 1321N1 was assessed. These agonists produced a rapid and transient increase of the intracellular Ca2+ concentration. When LPA was perfused before addition of EGF, the EGF-dependent Ca2+ transient was abrogated, whereas this was not observed when EGF preceded LPA addition. This inhibitory effect was not found for other EGF-mediated responses, e.g., activation of the mitogen-activated protein kinase cascade and cell proliferation, thus pointing to the existence of cross-talk between LPA and EGF for only a branch of EGF-induced responses. As 1321N1 cells expressed mRNA encoding the LPA receptors endothelial differentiation gene (Edg)-2, Edg-4, and Edg-7 and as sphingosine 1-phosphate did not interfere with LPA signaling, Edg-2, Edg-4, and/or Edg-7 could be considered as the LPA receptors mediating the aforementioned cross-talk. Attempts to address the biochemical mechanism involved in the cross-talk between the receptors were conducted by the immunoprecipitation approach using antibodies reacting with the EGF receptor (EGFR), phosphotyrosine, phospholipase Cγ (PLCγ)-1, and G(αi) protein. LPA was found to induce coupling of PLCγ-1 to the EGFR by a mechanism involving a G(αi) protein, in the absence of tyrosine phosphorylation of both PLCγ and the EGFR. These data show a cross-talk between LPA and EGF limited to a branch of EGFR-mediated signaling, which may be explained by a LPA-induced, G(αi)-protein-mediated translocation of PLCγ-1 to EGFR in the absence of detectable tyrosine phosphorylation of both proteins. | URI: | http://hdl.handle.net/10261/71773 | DOI: | 10.1046/j.1471-4159.2000.0751575.x | Identificadores: | doi: 10.1046/j.1471-4159.2000.0751575.x issn: 0022-3042 e-issn: 1471-4159 |
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