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Dynamics of Potyvirus Systemic Infection Establishment: Independent yet Cumulative Action of Primary Infection Sites

AuthorsLafforgue, Guillaume ; Tromas, Nicolas; Elena, Santiago F. ; Zwart, Mark P.
Nuclear polyhedrosis-Virus
Genetic bottlenecks
Issue DateDec-2012
PublisherAmerican Society for Microbiology
CitationJournal of Virology 86/23: 12912-12922 (2012)
AbstractIn the clinic, farm or field, for many viruses there is a high prevalence of mixed-genotype 4 infections, indicating that multiple virions have initiated infection and that there can be 5 multiple sites of primary infection within the same host. The dynamical process by which 6 multiple primary infection sites interact with each other and the host is poorly understood, 7 undoubtedly due to its high complexity. Here we attempt to unravel the basic interactions 8 underlying this process using a plant RNA virus, as removing the inoculated leaf can instantly 9 and rigorously eliminate all primary infection sites. Effective population size in the inoculated 10 leaf and time of removal of the inoculated leaf were varied in experiments, and it was found 11 that both factors positively influenced if the plant became systemically infected and what 12 proportion of cells in the systemic tissue were infected, as measured be flow cytometry. 13 Fitting of probabilistic models of infection to our data demonstrated that a null model in which 14 the action of each focus is independent of the presence of other foci was better supported 15 than a dependent action model. The cumulative effect of independently acting foci therefore 16 determined when plants became infected and how many individual cells were infected. 17 There was no evidence for interference between primary infection sites, which is surprising 18 given planar structure of leaves. By showing a simple null-model is supported, we 19 experimentally confirm – to our knowledge for the first time – the minimal components that 20 dictate interactions of a conspecific virus population establishing systemic infection.
Publisher version (URL)http://dx.doi:10.1128/JVI.02207-12
Appears in Collections:(IBMCP) Artículos
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