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dc.contributor.authorObeso Díaz, José María-
dc.contributor.authorMartínez Fernández, Beatriz-
dc.contributor.authorRodríguez González, Ana-
dc.contributor.authorGarcía Suárez, María Pilar-
dc.date.accessioned2008-09-10T11:01:16Z-
dc.date.available2008-09-10T11:01:16Z-
dc.date.issued2008-
dc.identifier.citationInternational Journal of Food Microbiology 128(2): 212–218 (2008)en_US
dc.identifier.issn0168-1605-
dc.identifier.urihttp://hdl.handle.net/10261/7147-
dc.description.abstractThe endolysin gene (lysH5) from the genome of the Staphylococcus aureus bacteriophage ΦH5 was cloned in Escherichia coli and characterized. The lysH5 gene encoded a protein (LysH5) whose calculated molecular mass and pI were 53.7 kDa and 8.7, respectively. Comparative analysis revealed that LysH5 significantly resembled other murein hydrolases encoded by staphylococcal phages. The modular organization of LysH5 comprised three putative domains, namely, CHAP (cysteine, histidine-dependent amidohydrolase/peptidase), amidase (l-muramoyl-l-alanine amidase), and SH3b (cell wall recognition). In turbidity reduction assays, the purified protein lysed bovine and human S. aureus, and human Staphylococcus epidermidis strains. Other bacteria belonging to different genera were not affected. The lytic activity was optimal at pH 7.0, 37˚C, and sensitive to high temperatures. The purified protein was able to kill rapidly S. aureus growing in pasteurized milk and the pathogen was not detected after 4 h of incubation at 37˚C. As far as we know, this is the first report to assess the antimicrobial activity of a phage endolysin which might be useful for novel biocontrol strategies in dairying.en_US
dc.description.sponsorshipThis research study was supported by grants AGL2006-03659/ALI from the Ministry of Education of Spain and PC06-009 from FICYT (Regional Government of Asturias). P.G. is a fellow of the Spanish Ministry of Education Ramón y Cajal Research Programme. J.O. is a fellow of the Spanish Ministry of Education (I3P Programme- CSIC).en_US
dc.format.extent380798 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsopenAccessen_US
dc.subjectEndolysinen_US
dc.subjectNatural antimicrobialsen_US
dc.subjectBiopreservationen_US
dc.subjectStaphylococcus aureusen_US
dc.titleLytic activity of the recombinant staphylococcal bacteriophage ΦH5 endolysin active against Staphylococcus aureus in milken_US
dc.typeartículoen_US
dc.identifier.doi10.1016/j.ijfoodmicro.2008.08.010-
dc.description.peerreviewedPeer revieweden_US
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)-
dc.contributor.funderMinisterio de Educación (España)-
dc.contributor.funderFundación para el Fomento en Asturias de la Investigación Científica Aplicada y la Tecnología-
dc.contributor.funderEuropean Commission-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003339es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100008430es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
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