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Degradation of 16S rRNA and attributes of viability of viable but nonculturable probiotic bacteria

AutorLahtinen, Sampo; Ahokoski, H.; Reinikainen, Johanna P.; Gueimonde Fernández, Miguel ; Nurmi, J.; Ouwehand, Arthur C.; Salminen, Seppo
Palabras clave16S rRNA
Probiotics
Quantitative real-time PCR
Viability
Viable but nonculturable
Fecha de publicación28-abr-2008
EditorSociety for Applied Microbiology
CitaciónLetters in Applied Microbiology 46(6): 693-698 (2008)
Resumen[Aims]To assess the stability of 16S rRNA of viable but nonculturable (VBNC) probiotics during storage when compared with different attributes of viability.
[Methods and Results] Levels of RNA of the probiotic strains Bifidobacterium longum 46, B. longum 2C and B. animalis subsp. lactis Bb-12 were monitored during storage in fermented and nonfermented foods. Cells which gradually lost their culturability in fermented products retained high level of rRNA, whereas rRNA of acid-killed control cells decreased at faster rate. Furthermore, the viability of B. longum 2C was monitored during storage by measuring changes in reductase activity, cytoplasmic membrane integrity and esterase activity using a flow cytometer. All of the culture-independent viability assays suggested that the cells remained viable during storage. In nonfermented media, the observed losses in culturability were smaller, and the changes in cell counts were comparable with the changes in rRNA levels.
[Conclusions] Viable but nonculturable probiotics maintain high levels of rRNA and retain properties of viable bacteria including reductase activity. Quantification of 16S rRNA complements culture-independent viability assays. Significance and Impact of the Study: Culture-independent
URIhttp://hdl.handle.net/10261/7139
DOI10.1111/j.1472-765X.2008.02374.x
ISSN0266-8254
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