English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/7114
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
 |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título : Molecular characterization of intrinsic and acquired antibiotic resistance in lactic acid bacteria and bifidobacteria
Autor : Ammor, Mohammed Salim ; Flórez García, Ana Belén ; H. A. M. van Hoek, Angela; González de los Reyes-Gavilán, Clara ; J.M. Aarts, Henk; Margolles Barros, Abelardo ; Mayo Pérez, Baltasar
Palabras clave : Bacterias ácido-lácticas
Multirresistencia
Bifidobacterias
Resistencia a los antibioticos
Fecha de publicación : 2008
Editor: S. Karger AG
Citación : Journal of Molecular Microbiology and Biotechnonoly 14(1-3): 6-15 (2008)
Resumen: The minimum inhibitory concentrations (MICs) of 6 different antibiotics (chloramphenicol, clindamycin, erythromycin, streptomycin, tetracycline and vancomycin) were determined for 143 strains of lactic acid bacteria and bifidobacteria using the Etest. Different MICs were found for different species and strains. Based on the distribution of these MIC values, most of the strains were either susceptible or intrinsically resistant to these antibiotics. However, the MIC range of some of these antibiotics showed a bimodal distribution, which suggested that some of the tested strains possess acquired antibiotic resistance. Screening for resistance genes was performed by PCR using specific primers, or using a DNA microarray with around 300 nucleotide probes representing 7 classes of antibiotic resistance genes. The genes identified encoded resistance to tetracycline [tet(M), tet(W), tet(O) and tet(O/W)], erythromycin and clindamycin [erm(B)] and streptomycin [aph(E) and sat(3)]. Internal portions of some of these determinants were sequenced and found to be identical to genes described in other bacteria. All resistance determinants were located on the bacterial chromosome, except for tet(M), which was identified on plasmids in Lactococcus lactis. The contribution of intrinsic multidrug transporters to the antibiotic resistance was investigated by cloning and measuring the expression of Bifidobacterium breve genes in L. lactis.
URI : http://hdl.handle.net/10261/7114
DOI: 10.1 159/000106077
ISSN: 1464-1801
Aparece en las colecciones: (IPLA) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
JMMB-Ammor-2008.pdf1,64 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.