Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/7077
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Gueimonde Fernández, Miguel | - |
dc.contributor.author | Noriega Pérez, Luis | - |
dc.contributor.author | Margolles Barros, Abelardo | - |
dc.contributor.author | González de los Reyes-Gavilán, Clara | - |
dc.date.accessioned | 2008-09-03T10:17:55Z | - |
dc.date.available | 2008-09-03T10:17:55Z | - |
dc.date.issued | 2006-10-10 | - |
dc.identifier.citation | Archives of Microbiology 187(2): 145–153 (2007) | en_US |
dc.identifier.issn | 0302-8933 | - |
dc.identifier.uri | http://hdl.handle.net/10261/7077 | - |
dc.description.abstract | Bifidobacterium longum can be isolated from human faeces, some strains being considered probiotics. B. longum NIZO B667 produces an exo-acting α-L-arabinofuranosidase, AbfB, previously purified by us, that releases L-arabinose from arabinan and arabinoxylan. This activity was subjected to two-seven-fold induction by L-arabinose, D-xylose, L-arabitol and xylitol and to repression by glucose. Maximum activity was obtained at 48 h incubation except for D-xylose that was at 24 h. High concentrations (200 mM) of L-arabitol also caused repression of the arabinofuranosidase. A unique band of activity showing the same migration pattern as the purified AbfB was found in zymograms of cell free extracts, indicating that the activity was likely due to this sole enzyme. The assessment of the influence of inducers and repressors on the activity of AbfB and on the expression of the abfB gene by real time PCR indicated that regulation was transcriptional. DNA amplifications using a pair of degenerated primers flanking an internal fragment within α-L-arabinofuranosidase genes of the family 51 of glycoside hydrolases evidenced that these enzymes are widespread in Bifidobacterium. The aminoacidic sequences of bifidobacteria included a fragment of four to six residues in the position 136-141 that was absent in other microorganisms | en_US |
dc.description.sponsorship | This work was financed by European Union FEDER funds and the Spanish Plan Nacional de I + D (project AGL2004-06088-C02-01/ALI). L. Noriega was the recipient of a predoctoral fellowship from Fundación para la investigación Científica y Técnica (FICYT, Asturias, Spain). M. Gueimonde was funded by a Juan de la Cierva postdoctoral contract from the Spanish Ministry of Education and Science. | en_US |
dc.format.extent | 481666 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | Springer Nature | en_US |
dc.rights | openAccess | en_US |
dc.subject | Bifidobacterias | en_US |
dc.subject | Probióticos | en_US |
dc.subject | Arabinofuranosidase | - |
dc.subject | Bifidobacterium | - |
dc.subject | Induction | - |
dc.title | Induction of α-L-arabinofuranosidase activity by monomeric carbohydrates in Bifidobacterium longum and ubiquity of encoding genes | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1007/s00203-006-0181-x | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://dx.doi.org/10.1007/s00203-006-0181-x | - |
dc.contributor.funder | European Commission | - |
dc.contributor.funder | Fundación para el Fomento en Asturias de la Investigación Científica Aplicada y la Tecnología | - |
dc.contributor.funder | Ministerio de Educación y Ciencia (España) | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100008430 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
Aparece en las colecciones: | (IPLA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
NoriegaArchives2007.pdf | 470,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
23
checked on 13-abr-2024
WEB OF SCIENCETM
Citations
22
checked on 29-feb-2024
Page view(s)
356
checked on 19-abr-2024
Download(s)
322
checked on 19-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.