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dc.contributor.authorSánchez García, Borja-
dc.contributor.authorChampomier-Vergès, Marie-Christine-
dc.contributor.authorCollado, María Carmen-
dc.contributor.authorAnglade, Patricia-
dc.contributor.authorBaraige, Fabienne-
dc.contributor.authorSanz, Yolanda-
dc.contributor.authorGonzález de los Reyes-Gavilán, Clara-
dc.contributor.authorMargolles Barros, Abelardo-
dc.contributor.authorZagorec, Monique-
dc.date.accessioned2008-09-02T08:27:27Z-
dc.date.available2008-09-02T08:27:27Z-
dc.date.issued2007-08-24-
dc.identifier.citationApplied and environmental microbiology 73 (20): 6450-6459 (2007)en_US
dc.identifier.issn0099-2240-
dc.identifier.urihttp://hdl.handle.net/10261/7049-
dc.description.abstractBifidobacteria are one of the main microbial inhabitants of the human colon. Usually administered in fermented dairy products as beneficial microorganisms, they have to overcome the acidic pH found in the stomach during the gastrointestinal transit to be able to colonize the lower parts of the intestine. The mechanisms underlying acid response and adaptation in Bifidobacterium longum biotype longum NCIMB 8809 and its mutant resistant to acid pH B. longum biotype longum 8809dpH, were studied. Comparison of protein maps, and protein identification by MALDI-TOF mass spectrometry analysis, allowed us to identify 9 different proteins whose production largely changed in the mutant strain. Furthermore, the production of 47 proteins was modulated by pH in one or both strains. These included general stress response chaperones, proteins involved in transcription and translation, as well as in the carbohydrate and nitrogen metabolism, among others. Significant differences in the levels of metabolic end-products and in the redox status of the cells were also detected between the wild type strain and its acid pH resistant mutant in response, or as a result of, adaptation to acid. Remarkably, the results of this work indicated that adaptation and response to low pH in B. longum biotype longum involves changes in the glycolytic flux and in the ability to regulate the internal pH. These changes were accompanied by a higher content of ammonium in the cytoplasm, likely coming from amino acid deamination, and a decrease of the bile salt hydrolase activityen_US
dc.description.sponsorshipThis work was financed by European Union FEDER funds and the Spanish Plan Nacional de I + D (project AGL2004-06727-C02-01/ALI and AGL2005-05788-C02-01/ALI). B. Sánchez was the recipient of a FPI predoctoral fellowship from the Spanish Ministerio de Ciencia y Tecnología and from the MICA department of the National Institute for Research in Agronomy (INRA).en_US
dc.format.extent379220 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.rightsopenAccessen_US
dc.subjectBifidobacteriasen_US
dc.subjectProteinasen_US
dc.titleLow pH adaptation and the acid tolerance response of Bifidobacterium longum biotype longumen_US
dc.typeartículoen_US
dc.identifier.doi10.1128/AEM.00886-07-
dc.description.peerreviewedPeer revieweden_US
dc.contributor.funderInstitut National de la Recherche Agronomique (France)-
dc.contributor.funderEuropean Commission-
dc.contributor.funderComisión Interministerial de Ciencia y Tecnología, CICYT (España)-
dc.contributor.funderMinisterio de Ciencia y Tecnología (España)-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100006488es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100006280es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100007273es_ES
dc.identifier.pmid17720838-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.openairetypeartículo-
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