Please use this identifier to cite or link to this item:
http://hdl.handle.net/10261/7049
Share/Export:
![]() ![]() |
|
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Title: | Low pH adaptation and the acid tolerance response of Bifidobacterium longum biotype longum |
Authors: | Sánchez García, Borja CSIC ORCID; Champomier-Vergès, Marie-Christine; Collado, María Carmen CSIC ORCID; Anglade, Patricia; Baraige, Fabienne; Sanz, Yolanda; González de los Reyes-Gavilán, Clara CSIC ORCID ; Margolles Barros, Abelardo CSIC ORCID; Zagorec, Monique | Keywords: | Bifidobacterias Proteinas |
Issue Date: | 24-Aug-2007 | Publisher: | American Society for Microbiology | Citation: | Applied and environmental microbiology 73 (20): 6450-6459 (2007) | Abstract: | Bifidobacteria are one of the main microbial inhabitants of the human colon. Usually administered in fermented dairy products as beneficial microorganisms, they have to overcome the acidic pH found in the stomach during the gastrointestinal transit to be able to colonize the lower parts of the intestine. The mechanisms underlying acid response and adaptation in Bifidobacterium longum biotype longum NCIMB 8809 and its mutant resistant to acid pH B. longum biotype longum 8809dpH, were studied. Comparison of protein maps, and protein identification by MALDI-TOF mass spectrometry analysis, allowed us to identify 9 different proteins whose production largely changed in the mutant strain. Furthermore, the production of 47 proteins was modulated by pH in one or both strains. These included general stress response chaperones, proteins involved in transcription and translation, as well as in the carbohydrate and nitrogen metabolism, among others. Significant differences in the levels of metabolic end-products and in the redox status of the cells were also detected between the wild type strain and its acid pH resistant mutant in response, or as a result of, adaptation to acid. Remarkably, the results of this work indicated that adaptation and response to low pH in B. longum biotype longum involves changes in the glycolytic flux and in the ability to regulate the internal pH. These changes were accompanied by a higher content of ammonium in the cytoplasm, likely coming from amino acid deamination, and a decrease of the bile salt hydrolase activity | URI: | http://hdl.handle.net/10261/7049 | DOI: | 10.1128/AEM.00886-07 | ISSN: | 0099-2240 |
Appears in Collections: | (IPLA) Artículos (IATA) Artículos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Borja pH 6450.pdf | 370,33 kB | Adobe PDF | ![]() View/Open |
Review this work
PubMed Central
Citations
41
checked on May 18, 2022
SCOPUSTM
Citations
144
checked on May 14, 2022
WEB OF SCIENCETM
Citations
141
checked on May 14, 2022
Page view(s)
334
checked on May 18, 2022
Download(s)
221
checked on May 18, 2022
Google ScholarTM
Check
Altmetric
Dimensions
Related articles:
WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.