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Labeling of Bifidobacterium longum Cells with 13C-Substituted Leucine for Quantitative Proteomic Analyses

AuthorsCouté, Yohann; Hernandez, Céline; Ron D., Appel; Sanchez, Jean-Charles; Margolles Barros, Abelardo
Issue Date29-Jun-2007
PublisherAmerican Society for Microbiology
CitationApplied and Environmental Microbiology 73(17): 5653-5656 (2007)
AbstractStable isotope labeling of amino acids in cell culture was used for Bifidobacterium longum. A comprehensive proteomic strategy was developed and validated by designing an appropriate semidefined medium that allows stable replacement of natural leucine by [13C6]leucine. Using this strategy, proteins having variations of at least 50% in their expression rates can be quantified with great confidence
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