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Two membrane proteins from Bifidobacterium breve UCC2003 constitute an ABC-type multidrug transporter

AutorMargolles Barros, Abelardo ; Flórez García, Ana Belén ; Moreno Muñoz, José Antonio; Van Sinderen, Douwe; González de los Reyes-Gavilán, Clara
Palabras claveBacteria láctica
Micrococcales
Streptococcaceae
Actinomycetes
Actinomycetales
Actinomycetaceae
Fecha de publicación8-ago-2008
EditorSociety for General Microbiology
CitaciónMicrobiology 152(12): 3497-3505 (2006)
ResumenIntrinsic resistance to drugs is one of the main determining factors in bacterial survival in the intestinal ecosystem. This is mediated by, among others, multidrug resistance (MDR) transporters, membrane proteins which extrude noxious compounds with very different chemical structures and cellular targets. Two genes from Bifidobacterium breve encoding hypothetical membrane proteins with a high homology with members of the ATP-binding cassette (ABC) family of multidrug efflux transporters, were expressed separately and jointly in Lactococcus lactis. Cells co-expressing both proteins exhibited enhanced resistance levels to the antimicrobials nisin and polymyxin B. Furthermore, the drug extrusion activity in membrane vesicles was increased when both proteins were co-expressed, compared to membranes in which the proteins were produced independently. Both proteins were co-purified from the membrane as a stable complex in a 1 :1 ratio. This is believed to be the first study of a functional ABC-type multidrug transporter in Bifidobacterium and contributes to our understanding of the molecular mechanisms underlying the capacity of intestinal bacteria to tolerate cytotoxic compounds
URIhttp://hdl.handle.net/10261/7021
DOI10.1099/mic.0.29097-0
ISSN1350-0872
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