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http://hdl.handle.net/10261/6990
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dc.contributor.author | Martín-Martín, B. | - |
dc.contributor.author | Nabokina, S. M. | - |
dc.contributor.author | Blasi, Juan | - |
dc.contributor.author | Lazo, Pedro A. | - |
dc.contributor.author | Mollinedo, Faustino | - |
dc.date.accessioned | 2008-08-27T14:55:57Z | - |
dc.date.available | 2008-08-27T14:55:57Z | - |
dc.date.issued | 2000-10 | - |
dc.identifier.citation | Blood 96: 2574-2583 (2000) | en_US |
dc.identifier.issn | 0006-4971 | - |
dc.identifier.uri | http://hdl.handle.net/10261/6990 | - |
dc.description.abstract | To understand the molecular basis of exocytosis in human neutrophils, the role of syntaxin 6 and SNAP-23 in neutrophil degranulation was examined. Human syntaxin 6 was cloned and identified as a 255-amino acid protein with a carboxyterminal transmembrane region and two coiled-coil domains. Syntaxin 6 was localized mainly in the plasma membrane of human resting neutrophils, whereas SNAP-23 was located primarily in the mobilizable tertiary and specific granules. SNAP-23 was translocated to the cell surface, colocalizing with syntaxin 6, on neutrophil activation. In vitro binding studies established that SNAP-23 binds to syntaxin 6. Coimmunoprecipitation assays indicated that SNAP-23 interacts with syntaxin 6 in vivo, and this interaction was dramatically increased on neutrophil activation. Antibodies against SNAP-23 inhibited Ca11 and GTP-g-S–induced exocytosis of CD67-enriched specific granules, but they hardly affected exocytosis of the CD63-enriched azurophilic granules, when introduced into electropermeabilized neutrophils. Anti–syntaxin 6 antibodies prevented exocytosis of both CD67- and CD63-enriched granules in electropermeabilized neutrophils. These data show that syntaxin 6 and SNAP-23 are involved in human neutrophil exocytosis, demonstrating that vesicle SNAP receptor- target SNAP receptor (v-SNARE– t-SNARE) interactions modulate neutrophil secretion. Syntaxin 6 acts as a target for secretion of specific and azurophilic granules, whereas SNAP-23 mediates specific granule secretion. | en_US |
dc.description.sponsorship | Supported by grant PB95-0713 from the Dirección General de Investigación Científica y Técnica (DGICYT), grant FIS98/0313 from the Fondo de Investigación Sanitaria, and grant 08.1/0004/98 from the Comunidad de Madrid | en_US |
dc.format.extent | 380876 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | American Society of Hematology | en_US |
dc.rights | closedAccess | en_US |
dc.subject | SNAP23 | en_US |
dc.subject | Syntaxin | en_US |
dc.title | Involvement of SNAP-23 and syntaxin 6 in human neutrophil exocytosis | en_US |
dc.type | artículo | en_US |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.languageiso639-1 | en | - |
item.grantfulltext | none | - |
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