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Title

Bioluminescence imaging of calcium oscillations inside intracellular organelles

AuthorsVillalobos, Carlos ; Alonso, María Teresa ; García-Sancho, Javier
KeywordsBioluminescence imaging
Aequorin
Herpes simplex virus
Calcium oscillations
Mitochondria
Nucleus
Issue Date2009
PublisherSpringer
CitationBioluminescence: 203-214 (2009)
SeriesMethods in Molecular Biology
574
AbstractCa2+ oscillations inside intracellular organelles are important for regulation of functions such as gene expression at the nucleus, respiration at mitochondria or protein processing at the endoplasmic reticulum. Targeted aequorins are excellent calcium probes for subcellular analysis, but single-cell imaging has proven difficult because of low light yield. Here we describe a procedure that combines virus-based expression of targeted aequorins with photon-counting imaging. This methodology allows real-time resolution of changes of cytosolic, mitochondrial or nuclear Ca2+ signals at the single-cell level.
DescriptionCapítulo 17.-- Editores: Preston B. Rich; Christelle Douillet.-- El pdf es la versión pre-print.
Publisher version (URL)http://dx.doi.org/10.1007/978-1-60327-321-3_17
URIhttp://hdl.handle.net/10261/69837
DOI10.1007/978-1-60327-321-3_17
ISBN978-1-60327-320-6
Appears in Collections:(IBGM) Libros y partes de libros
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