Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/6873
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Sánchez García, Borja | - |
dc.contributor.author | González de los Reyes-Gavilán, Clara | - |
dc.contributor.author | Margolles Barros, Abelardo | - |
dc.date.accessioned | 2008-08-22T11:11:05Z | - |
dc.date.available | 2008-08-22T11:11:05Z | - |
dc.date.issued | 2006-06-08 | - |
dc.identifier.citation | Environmental Microbiology 8(10): 1825-1833 (2006) | en_US |
dc.identifier.issn | 1462-2912 | - |
dc.identifier.uri | http://hdl.handle.net/10261/6873 | - |
dc.description.abstract | Adaptation and tolerance to bile stress are important factors for the survival of bifidobacteria in the intestinal tract. Bifidobacterium animalis is a probiotic microorganism which has been largely applied in fermented dairy foods due to its technological properties and its health-promoting effects for humans. The effect of the presence of bile on the activity and expression of F1F0-ATPase, the pool of ATP and the intracellular pH of B. animalis IPLA 4549 and its mutant with acquired resistance to bile B. animalis 4549dOx was determined. The bile-resistant mutant tolerated the acid pH better than its parent strain. Bile induced the expression of the F1F0-ATPase and increased the membrane-bound H+-ATPase activity, in both parent and mutant strains. In acidic conditions (pH 5.0), the expression and the activity of this enzyme were higher in the mutant than in the parent strain when cells were grown in the absence of bile. Total ATP content was higher for the mutant in the absence of bile, whereas the presence of bile induced a decrease of intracellular ATP levels, which was much more pronounced for the parent strain. At pH 4.0, and independently on the presence or absence of bile, the mutant showed a higher intracellular pH than its parent strain. These findings suggest that the bile-adapted B. animalis strain is able to tolerate bile by increasing the intracellular ATP reserve, and by inducing proton pumping by the F1F0-ATPase, therefore tightly regulating the internal pH, and provide a link between the physiological state of the cell and the response to bile | en_US |
dc.description.sponsorship | This work was financed by European Union FEDER funds and the Spanish Plan Nacional de I + D (AGL2004-06727-C02-01/ALI). B.S. was the recipient of a FPI predoctoral fellowship from the Spanish Ministerio de Educación y Ciencia. | en_US |
dc.format.extent | 222479 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | Society for Applied Microbiology | en_US |
dc.publisher | Blackwell Publishing | - |
dc.rights | openAccess | en_US |
dc.subject | Bifidobacteria | en_US |
dc.title | The F1F0-ATPase of Bifidobacterium animalis is involved in bile tolerance | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1111/j.1462-2920.2006.01067.x | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://dx.doi.org/10.1111/j.1462-2920.2006.01067.x | - |
dc.identifier.e-issn | 1462-2920 | - |
dc.contributor.funder | European Commission | - |
dc.contributor.funder | Comisión Interministerial de Ciencia y Tecnología, CICYT (España) | - |
dc.contributor.funder | Ministerio de Educación y Ciencia (España) | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100007273 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
item.openairetype | artículo | - |
Aparece en las colecciones: | (IPLA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
ATPasaB animalisEM2006.pdf | 217,26 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
81
checked on 22-abr-2024
WEB OF SCIENCETM
Citations
81
checked on 21-feb-2024
Page view(s)
441
checked on 24-abr-2024
Download(s)
461
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.