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Expression of interferon-γ receptors on murine oligodendrocytes and its regulation by cytokines and mitogens

AuthorsTorres, Concepción; Aránguez, Isabel; Rubio, Nazario CSIC ORCID
Issue Date1995
PublisherBlackwell Publishing
CitationImmunology 86: 250-255 (1995)
AbstractInterferon-γ (IFN-γ) is a cytokine known to exert an important immunological role on astrocytes and oligodendrocytes. As a receptor for IFN-γ has been demonstrated on murine astrocytes, we have searched for a specific receptor on the cell surface of pure mouse oligodendrocytes maintained in tissue culture. Using recombinant murine IFN-γ labelled with 125I, we have established the basic physicochemical parameters of the binding. A single receptor was found with a K(d) of 1 x 10-9 M. The number of receptors per cell was 3000-4000 and its molecular weight, as determined by cross-linking experiments, is 87 000. The binding of IFN-γ to its oligodendrocyte receptor is saturable, specific and temperature-dependent. The receptor-IFN-γ complex is quickly endocytosed at 37° (the half-time of maximal internalization is around 1 min). Some cytokines, such as interleukin-1α and interleukin-6, up-regulated the expression of the oligodendrocyte receptor, but others, such as tumour necrosis factor-α, did not. A dramatic increase in receptor expression is induced by lipopolysaccharide but it is not detectable after treatment with concanavalin A.
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