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Combined ATR-SEIRAS and EC-STM study of the immobilization of laccase on chemically modified Au electrodes

AuthorsVaz Domínguez, C. ; Pita, Marcos ; López de Lacey, Antonio ; Shleev, Sergey; Cuesta, Angel
Issue Date2012
PublisherAmerican Chemical Society
CitationJournal of Physical Chemistry C 116(31): 16532-16540 (2012)
AbstractThe successive steps of laccase immobilization on chemically modified Au electrodes were monitored using ATR-SEIRAS and in situ STM. Successful covalent immobilization of the enzyme on Au electrodes modified by a mixed aminophenyl-mercaptohexanol adlayer and on Au electrodes modified by a 4-aminothiophenyl SAM via a Schiff base reaction followed by the formation of amide bonds is revealed by the emergence of the corresponding bands in the ATR-SEIRA spectra, and an enzyme coverage on aminophenyl-mercaptohexanol- modified Au electrodes of about (7.27 ± 1.93) × 10 11 laccase units per cm 2 was calculated from STM images. The small differences between the ATR-SEIRA spectra of the enzyme immobilized on aminophenyl-mercaptohexanol-modified Au electrodes and the ATR-SEIRA spectra of the enzyme immobilized on 4-aminothiophenyl-modified Au electrodes are attributed to a different orientation of the immobilized enzyme due to the presence on the surface of aminophenyl-mercaptohexanol-modified Au electrodes of OH functional groups that favor an orientation of laccase with the Cu T1 center of the enzyme facing the electrode surface, thus, allowing a high activity for direct electrocatalysis of the ORR at low overpotentials. © 2012 American Chemical Society.
Publisher version (URL)http://doi.org/10.1021/jp303818p
Identifiersdoi: 10.1021/jp303818p
issn: 1932-7447
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