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Título: | Uptake of sucrose by Saccharomyces cerevisiae |
Autor: | Santos de Dios, Eugenio CSIC ORCID CVN ; Rodríguez, Luis; Elorza, M. Victoria; Sentandreu, Rafael | Fecha de publicación: | 1982 | Editor: | Elsevier | Citación: | Archives of Biochemistry and Biophysics 216(2): 652-660 (1982) | Resumen: | Sucrose transport has been shown to occur in several Suc− and Suc+Saccharomyces cerevisiae strains as an energy-dependent process. Assay conditions have been established to avoid both extra- and intracellular hydrolysis of the disaccharide thus allowing the identification of sucrose as such inside the cell immediately after the uptake; acid pH values (4.0–5.0) were optimal for transport although significant uptake was also detected at neutral pH. Transport of sucrose was not dependent on ATP and seemed to be driven by protonmotive force supplied by the electrochemical gradient of protons across the plasma membrane. The actual symport of protons along with sucrose was directly detected by continuous pH measurement of the reaction mixtures and the initial rate of proton movement in the symport process was determined. KC1 inhibited transport of sucrose suggesting that exit of K+ ions might well be involved in maintaining the electroneutrality of the process. On the other hand, NaCl stimulated transport by 50% in our experimental conditions. The specificity of sucrose transport was also tested using different disaccharides. | URI: | http://hdl.handle.net/10261/67357 | DOI: | 10.1016/0003-9861(82)90255-7 | Identificadores: | doi: 10.1016/0003-9861(82)90255-7 issn: 0003-9861 |
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