English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/67225
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Functionally homologous DNA replication genes in fission and budding yeast

AuthorsSánchez, Mar; Calzada, Arturo; Bueno, Avelino
Issue Date1999
PublisherCompany of Biologists
CitationJournal of Cell Science 112(14): 2381-2390 (1999)
AbstractThe cdc18+ gene of the fission yeast Schizosaccharomyces pombe is involved in the initiation of DNA replication as well as in coupling the S phase to mitosis. In this work, we show that the Saccharomyces cerevisiae CDC6 gene complements cdc18-K46 ts and cdc18 deletion mutant S. pombe strains. The budding yeast gene suppresses both the initiation and the checkpoint defects associated with the lack of cdc18+. The Cdc6 protein interacts in vivo with Cdc2 kinase complexes. Interestingly, Cdc6 is an in vitro substrate for Cdc13/Cdc2 and Cig1/Cdc2, but not for Cig2/Cdc2-associated kinases. Overexpression of Cdc6 in fission yeast induces multiple rounds of S-phase in the absence of mitosis and cell division. This CDC6-dependent continuous DNA synthesis phenotype is independent of the presence of a functional cdc18+ gene product and, significantly, requires only Cig2/Cdc2-associated kinase activity. Finally, these S. pombe over-replicating cells do not require any protein synthesis other than that of Cdc6. Our data strongly suggest that CDC6 and cdc18+ are functional homologues and also support the idea that controls restricting genome duplication diverge in fission and budding yeasts.
Publisher version (URL)http://jcs.biologists.org/content/112/14/2381.abstract
Identifiersissn: 0021-9533
e-issn: 1477-9137
Appears in Collections:(IBMCC) Artículos
(IMB) Artículos
Files in This Item:
File Description SizeFormat 
Functionally homologous.pdf448,02 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.