English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/67055
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


IL-1, IL-2 and IFN-γ production by Theiler's virus-induced encephalomyelitic SJL/J mice

AuthorsRubio, Nazario CSIC ORCID; Torres, Concepción
Issue Date1991
PublisherBlackwell Publishing
CitationImmunology 74: 284- 289 (1991)
AbstractInterleukins have been postulated to exert an important modulatory and recruiting role in the Theiler's murine encephalomyelitis virus (TMEV)-induced demyelination of the central nervous system (CNS) in SJL/J mice. Using bio- and radioimmunoassays, we have detected and quantified some of the interleukins suspected to play a role in this immune-mediated process. The interleukins 1 and 2 (IL-1, IL-2) and interferon-gamma (IFN-γ) have been measured in homogenates from the CNS and sera from infected animals, as well as in supernatants from antigen-specific in vitro-stimulated spleen and meningeal cells. IL-1 was detected both in CNS tissue homogenates (approximately 20-40 fmol/ml) and in splenic cultures (200 U/ml). IL-2 was detected only after TMEV-specific antigenic stimulation in spleen cultures (approximately 120 U/ml). In vitro, IL-2 and IL-1 synthesis appear in a dose- and time-dependent manner. IFN-γ could not be found in any case. The precise nature of IL-1 and IL-2 activity was further assessed by HPLC. The above results strongly indicate the presence of functionally active macrophages in the CNS infiltrates of cells triggering this autoaggressive immune process. In addition, we propose a central role for IL-1 in augmenting the intracerebral immune response leading to the inflammatory demyelination induced by TMEV.
Appears in Collections:(IC) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.