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http://hdl.handle.net/10261/67044
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Torrado, Ana | - |
dc.contributor.author | Vázquez, José Antonio | - |
dc.contributor.author | Prieto, Miguel Ángel | - |
dc.contributor.author | Fuciños, Pablo | - |
dc.contributor.author | Montemayor, María I. | - |
dc.contributor.author | Pastrana, Lorenzo | - |
dc.contributor.author | González Fernández, Pilar | - |
dc.contributor.author | Murado García, Miguel Anxo | - |
dc.date.accessioned | 2013-02-20T13:08:58Z | - |
dc.date.available | 2013-02-20T13:08:58Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Journal of Chemical Technology and Biotechnology 88(2): 226-236 (2013) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/67044 | - |
dc.description | 11 páginas, 6 figuras, 4 tablas | es_ES |
dc.description.abstract | BACKGROUND: The high oxygen availability in solid-state cultures makes them especially suitable for fungal enzyme production. Glycogen-rich mussel processing wastewaters have been used successfully as substrates for amylase production by Aspegillus oryzae in solid-state cultures supported in polyurethane foam. The aim of this work was to study the fed-batch mode in a scalable solid-state bioreactor to extend the productive period and obtain high amylase production. Culture salinization due to the NaCl content of these wastewaters is the main drawback. RESULTS: Evaporation of the excess liquid added during feeding led to progressive salt accumulation. The effect of culture salinization on amylase production was analysed and mathematically modelled, and the IC50 (65.4 g L−1 of NaCl) was calculated. An optimum operation mode for this bioreactor was designed that included foam extrusion for removing the incubated medium, washing and a final recharge with fresh medium every 72 h of incubation. This procedure kept the salt concentration under IC50 and increased the amylase production from 3000 to 12 000 UE g−1. CONCLUSION: An operating mode with intermittent extrusions and washings of the support between feedings was found to be an appropriate procedure for preventing the accumulation of inhibitory compounds in fed-batch solid-state cultures. © 2012 Society of Chemical Industry | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | John Wiley & Sons | es_ES |
dc.rights | closedAccess | es_ES |
dc.subject | Amylase production | es_ES |
dc.subject | Mussel processing wastewaters | es_ES |
dc.subject | Solid-state | es_ES |
dc.subject | Salinization | es_ES |
dc.subject | Solid-state bioreactor | es_ES |
dc.subject | Fed-batch | es_ES |
dc.subject | Weibull equation | es_ES |
dc.subject | SSC | es_ES |
dc.subject | SSF | es_ES |
dc.title | Amylase production by Aspergillus oryzae in a solid-state bioreactor with fed-batch operation using mussel processing wastewaters as feeding medium | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1002/jctb.3813 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1002/jctb.3813 | es_ES |
dc.identifier.e-issn | 1097-4660 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
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