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dc.contributor.authorTorrado, Ana-
dc.contributor.authorVázquez, José Antonio-
dc.contributor.authorPrieto, Miguel Ángel-
dc.contributor.authorFuciños, Pablo-
dc.contributor.authorMontemayor, María I.-
dc.contributor.authorPastrana, Lorenzo-
dc.contributor.authorGonzález Fernández, Pilar-
dc.contributor.authorMurado García, Miguel Anxo-
dc.date.accessioned2013-02-20T13:08:58Z-
dc.date.available2013-02-20T13:08:58Z-
dc.date.issued2013-
dc.identifier.citationJournal of Chemical Technology and Biotechnology 88(2): 226-236 (2013)es_ES
dc.identifier.urihttp://hdl.handle.net/10261/67044-
dc.description11 páginas, 6 figuras, 4 tablases_ES
dc.description.abstractBACKGROUND: The high oxygen availability in solid-state cultures makes them especially suitable for fungal enzyme production. Glycogen-rich mussel processing wastewaters have been used successfully as substrates for amylase production by Aspegillus oryzae in solid-state cultures supported in polyurethane foam. The aim of this work was to study the fed-batch mode in a scalable solid-state bioreactor to extend the productive period and obtain high amylase production. Culture salinization due to the NaCl content of these wastewaters is the main drawback. RESULTS: Evaporation of the excess liquid added during feeding led to progressive salt accumulation. The effect of culture salinization on amylase production was analysed and mathematically modelled, and the IC50 (65.4 g L−1 of NaCl) was calculated. An optimum operation mode for this bioreactor was designed that included foam extrusion for removing the incubated medium, washing and a final recharge with fresh medium every 72 h of incubation. This procedure kept the salt concentration under IC50 and increased the amylase production from 3000 to 12 000 UE g−1. CONCLUSION: An operating mode with intermittent extrusions and washings of the support between feedings was found to be an appropriate procedure for preventing the accumulation of inhibitory compounds in fed-batch solid-state cultures. © 2012 Society of Chemical Industryes_ES
dc.language.isoenges_ES
dc.publisherJohn Wiley & Sonses_ES
dc.rightsclosedAccesses_ES
dc.subjectAmylase productiones_ES
dc.subjectMussel processing wastewaterses_ES
dc.subjectSolid-statees_ES
dc.subjectSalinizationes_ES
dc.subjectSolid-state bioreactores_ES
dc.subjectFed-batches_ES
dc.subjectWeibull equationes_ES
dc.subjectSSCes_ES
dc.subjectSSFes_ES
dc.titleAmylase production by Aspergillus oryzae in a solid-state bioreactor with fed-batch operation using mussel processing wastewaters as feeding mediumes_ES
dc.typeartículoes_ES
dc.identifier.doi10.1002/jctb.3813-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1002/jctb.3813es_ES
dc.identifier.e-issn1097-4660-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
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