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Non-conventional yeasts as hosts for heterologous protein production

AuthorsDomínguez Olavarri, Ángel; Fermiñán, Encarnación ; Sánchez, Manuel; Gónzalez, Francisco J.; Pérez-Campo, Flor Maria; García, Susana; Herrero, Ana B.; San Vicente, Avelino; Cabello, Juan ; Prado, Marciano; Choupina, Altino; Fernández-Lago, Luis; López, M. Carmen
Issue Date1998
Sociedad Española de Microbiología
CitationInternational Microbiology 1(2): 131-142 (1998)
AbstractYeasts are an attractive group of lower eukaryotic microorganisms, some of which are used in several industrial processes that include brewing, baking and the production of a variety of biochemical compounds. More recently, yeasts have been developed as host organisms for the production of foreign (heterologous) proteins. Saccharomyces ccrevisiae has usually been the yeast of choice, but an increasing number of alternative non-Saccharomyces yeasts has now become accessible for modern molecular genetics techniques. Some of them exhibit certain favourable traits such as high-level secretion or very strong and tightly regulated promoters, offering significant advantages over traditional bakers' yeast. In the present work, the current status of Kluyveromyces lactis, Yarrowia lipolytica, Hansennla polymorpha and Picliia pastoris (the best-known alternative yeast systems) is reviewed. The advantages and limitations of these systems are discussed in relation to S. cerevisiae. © Springer-Verlag 1998.
DescriptionCreative Commons-Attribution-Non-Commercial-Share Alike 3.0 Spain.-- et al.
Publisher version (URL)http://www.im.microbios.org/02june98/08%20Dominguez.pdf
Identifiersissn: 1139-6709
e-issn: 1618-1905)
Appears in Collections:(IMB) Artículos
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