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dc.contributor.authorMatamoros Galindo, Manuel Ángel-
dc.contributor.authorFernández, Nieves-
dc.contributor.authorWienkoop, Stefanie-
dc.contributor.authorLoscos Aranda, Jorge-
dc.contributor.authorSaiz Rodríguez, Ana-
dc.identifier.citationMatamoros M, Fernández-García N, Wienkoop S, Loscos J, Saiz A. Mitochondria are an early target of oxidative modifications in senescing legume nodules. New Phytologist 197 (3): 873-885 (2013)es_ES
dc.description39 Pags., 4 Tabls., 8 Figs. The definitive version is available at: http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1469-8137es_ES
dc.description.abstractLegume nodule senescence is a poorly understood process involving a decrease in N2 fixation and an increase in proteolytic activity. Some physiological changes during nodule aging have been reported, but scarce information is available at the subcellular level. Biochemical, immunological and proteomic approaches were used to provide insight into the effects of aging on the mitochondria and cytosol of nodule host cells. In the mitochondria, the oxidative modification of lipids and proteins was associated with a marked decline in glutathione, a reduced capacity to regenerate ascorbate, and upregulation of alternative oxidase and manganese superoxide dismutase. In the cytosol, there were consistent reductions in the protein concentrations of carbon metabolism enzymes, inhibition of protein synthesis and increase in serine proteinase activity, disorganization of cytoskeleton, and a sharp reduction of cytosolic proteins, but no detectable accumulation of oxidized molecules. We conclude that nodule mitochondria are an early target of oxidative modifications and a likely source of redox signals. Alternative oxidase and manganese superoxide dismutase may play important roles in controlling ROS concentrations and the redox state of mitochondria. The finding that specific methionine residues of a cytosolic glutamine synthetase isoform are sulfoxidized suggests a regulatory role of this enzyme in senescing nodules.es_ES
dc.description.sponsorshipThis work was funded by Ministerio de Ciencia e Innovación-Fondo Europeo de Desarrollo Regional (AGL2011-24524) and Gobierno de Aragón-Fondo Social Europeo (group A53).es_ES
dc.publisherJohn Wiley & Sonses_ES
dc.subjectnodule senescencees_ES
dc.subjectoxidative damagees_ES
dc.subjectPhaseolus vulgaris (bean)es_ES
dc.titleMitochondria are an early target of oxidative modifications in senescing legume noduleses_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderEuropean Commission-
dc.contributor.funderGobierno de Aragón-
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