Please use this identifier to cite or link to this item:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Mitochondria are an early target of oxidative modifications in senescing legume nodules

AuthorsMatamoros Galindo, Manuel Ángel CSIC ORCID ; Fernández, Nieves CSIC ORCID; Wienkoop, Stefanie; Loscos Aranda, Jorge CSIC ORCID; Saiz Rodríguez, Ana CSIC
nodule senescence
oxidative damage
Phaseolus vulgaris (bean)
Issue DateFeb-2013
PublisherJohn Wiley & Sons
CitationMatamoros M, Fernández-García N, Wienkoop S, Loscos J, Saiz A. Mitochondria are an early target of oxidative modifications in senescing legume nodules. New Phytologist 197 (3): 873-885 (2013)
AbstractLegume nodule senescence is a poorly understood process involving a decrease in N2 fixation and an increase in proteolytic activity. Some physiological changes during nodule aging have been reported, but scarce information is available at the subcellular level. Biochemical, immunological and proteomic approaches were used to provide insight into the effects of aging on the mitochondria and cytosol of nodule host cells. In the mitochondria, the oxidative modification of lipids and proteins was associated with a marked decline in glutathione, a reduced capacity to regenerate ascorbate, and upregulation of alternative oxidase and manganese superoxide dismutase. In the cytosol, there were consistent reductions in the protein concentrations of carbon metabolism enzymes, inhibition of protein synthesis and increase in serine proteinase activity, disorganization of cytoskeleton, and a sharp reduction of cytosolic proteins, but no detectable accumulation of oxidized molecules. We conclude that nodule mitochondria are an early target of oxidative modifications and a likely source of redox signals. Alternative oxidase and manganese superoxide dismutase may play important roles in controlling ROS concentrations and the redox state of mitochondria. The finding that specific methionine residues of a cytosolic glutamine synthetase isoform are sulfoxidized suggests a regulatory role of this enzyme in senescing nodules.
Description39 Pags., 4 Tabls., 8 Figs. The definitive version is available at:
Publisher version (URL)
Appears in Collections:(EEAD) Artículos
(CEBAS) Artículos

Files in This Item:
File Description SizeFormat
BecanaM_NewPhytol_2013.pdf2,28 MBAdobe PDFThumbnail
Show full item record
Review this work


checked on Nov 23, 2021

Google ScholarTM




WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.