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Immunoblotting patterns in the serodiagnosis of aspergilloma: Antibody response to the 90 kDaAspergillus fumigatus antigen

AuthorsLópez-Medrano, R.; Ovejero, M. C.; Calera, José Antonio ; Puente, P.; Leal, Fernando
Issue Date1996
CitationEuropean Journal of Clinical Microbiology and Infectious Diseases 15(2): 146-152 (1996)
AbstractAt present there are no accepted criteria to assess the usefulness of Western blot assays for the serodiagnosis of aspergilloma. An Aspergillus fumigatus cytosolic fraction complex (CFC) composed of four proteins (p90, p60, p40, and p37) has been identified. The usefulness of Western blotting with CFC antigens for the serodiagnosis of aspergilloma was evaluated in 25 patients with well-established diagnoses and in 94 controls. The most consistently reactive antigen was p90 (92% of patients with aspergilloma), followed by p40 (76%) and the entire CFC taken together (76%). With these data, interpretive criteria for positive and negative immunoblots were established, with p90 indicated as a helpful marker of aspergilloma.
Identifiersdoi: 10.1007/BF01591488
issn: 0934-9723
e-issn: 1435-4373
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