Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/66538
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Aibel, Lani | - |
dc.contributor.author | Martín-Zanca, Dionisio | - |
dc.contributor.author | Pérez González, Pilar | - |
dc.contributor.author | Chao, Moses V. | - |
dc.date.accessioned | 2013-02-13T10:16:19Z | - |
dc.date.available | 2013-02-13T10:16:19Z | - |
dc.date.issued | 1998 | - |
dc.identifier | doi: 10.1002/(SICI)1097-4547(19981101)54:3<424::AID-JNR13>3.0.CO;2-6 | - |
dc.identifier | issn: 0360-4012 | - |
dc.identifier | e-issn: 1097-4547 | - |
dc.identifier.citation | Journal of Neuroscience Research 54(3): 424-431 (1998) | - |
dc.identifier.uri | http://hdl.handle.net/10261/66538 | - |
dc.description.abstract | Nerve growth factor (NGF) initiates its biological effects by promoting the dimerization and activation of the tyrosine kinase receptor TrkA. The requirements for NGF signaling through the TrkA receptor have been defined extensively from studies in immortalized cells, involving transfection of NIH 3T3, COS, and PC12 cells. In the present study, we tested the effects of extracellular and intracellular mutations of TrkA after DNA-mediated transfection in primary cultures of embryonic day 17 hippocampal neurons. We found that the action of the TrkA receptor on neuronal differentiation depends on specific motifs in the extracellular domain and on tyrosine 490 (Y490), the site for SHC protein binding. In contrast with previous observations in a PC12 background, a mutation in the SHC Y490 binding site in TrkA resulted in a loss of NGF-dependent process formation. These results indicate that tyrosine 490 is necessary for neurite outgrowth in hippocampal neurons. Moreover, a constitutively active form of TrkA did not give enhanced responsiveness in hippocampal neurons, indicating that the behavior of TrkA receptors in primary neuronal cells is distinct from that of other cell types. | - |
dc.description.sponsorship | This work was supported by the NIH (NS21072). | - |
dc.language.iso | eng | - |
dc.publisher | Wiley-Blackwell | - |
dc.rights | closedAccess | - |
dc.title | Functional expression of TrkA receptors in hippocampal neurons | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1002/(SICI)1097-4547(19981101)54:3<424::AID-JNR13>3.0.CO;2-6 | - |
dc.date.updated | 2013-02-13T10:16:19Z | - |
dc.description.version | Peer Reviewed | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.languageiso639-1 | en | - |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairetype | artículo | - |
Aparece en las colecciones: | (IMB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
15
checked on 20-abr-2024
Page view(s)
322
checked on 24-abr-2024
Download(s)
117
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.