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dc.contributor.authorGarcía Rodríguez, Patricia-
dc.contributor.authorGarcía, Ignacio-
dc.contributor.authorSánchez Martín, Yolanda-
dc.identifierdoi: 10.1534/genetics.108.094839-
dc.identifierissn: 0016-6731-
dc.identifiere-issn: 1943-2631-
dc.identifier.citationGenetics 181(4): 1321-1334 (2009)-
dc.description.abstractSchizosaccharomyces pombe Rho1p is essential, directly activates β-1,3-glucan synthase, and participates in the regulation of morphogenesis. In S. pombe, Rho1p is activated by at least three guanine nucleotide exchange factors (GEFs): Rgf1p, Rgf2p, and Rgf3p. In this study we show that Rgf2p is a Rho1p GEF required for sporulation. The rgf2+ deletion did not affect forespore membrane formation and the nuclei were encapsulated properly. However, the mutant ascospores appeared dark and immature. The rgf2Δ zygotes were not able to release the ascospores spontaneously, and the germination efficiency was greatly reduced compared to wild-type (wt) spores. This phenotype resembles that of the mutants in bgs2+, which encodes a sporulation-specific glucan synthase subunit. In fact, glucan synthase activity was diminished in sporulating rgf2Δ diploids. Rgf2p also plays a role in β-glucan biosynthesis during vegetative growth. Overexpression of rgf2+ specifically increased GTP-bound Rho1p, caused changes in cell morphology, and elicited an increase in β-1,3-glucan synthase activity. Moreover, the simultaneous disruption of rgf1+ and rgf2+ was lethal and both Rgf1p and Rgf2p were able to partially substitute for each other. Our results suggest that Rgf1p and Rgf2p are alternative GEFs with an essential overlapping function in Rho1p activation during vegetative growth. Copyright © 2009 by the Genetics Society of America.-
dc.description.sponsorshipP.G. and I.G. were supported by a fellowship from the Junta de Castilla y León. G.R. was supported by a fellowship from the Basque government. This work was supported by grants BFU2005-01557 and BFU2008-00963 from the Comisión Interministerial de Ciencia y Tecnología, Spain and SA008A07 andGR231 from the junta de Castilla y León.-
dc.publisherGenetics Society of America-
dc.titleFission yeast Rgf2p is a Rho1p guanine nucleotide exchange factor required for spore wall maturation and for the maintenance of cell integrity in the absence of Rgf1p-
dc.description.versionPeer Reviewed-
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