Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/66431
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Expression of the genes coding for the xylanase Xys1 and the cellulase Cel1 from the straw-decomposing Streptomyces halstedii JM8 cloned into the amino-acid producer Brevibacterium lactofermentum ATCC13869 |
Autor: | Díaz, Margarita CSIC ORCID; Fernández-Ábalos, José M. CSIC; Santamaría, Ramón I. CSIC ORCID | Fecha de publicación: | 2001 | Editor: | Springer Nature | Citación: | Archives of Microbiology 177(1): 91-97 (2001) | Resumen: | The xylanase ( xysA) and the cellulase ( celA1) genes from Streptomyces halstedii JM8 were cloned into Escherichia coli/ Brevibacterium lactofermentum shuttle vectors and successfully expressed in both hosts when placed downstream from the kanamycin resistance promoter (Pkan) from Tn 5 but not when under the control of their own promoters. Xylanase was secreted into the culture media of B. lactofermentum by removal of the same leader peptide as is removed in S. halstedii. The main difference between the production of xylanase by Streptomyces and corynebacteria was the low level of processing of the mature extracellular xylanase by B. lactofermentum, probably due to the lack of protease activity in this microorganism. | URI: | http://hdl.handle.net/10261/66431 | DOI: | 10.1007/s00203-001-0365-3 | Identificadores: | doi: 10.1007/s00203-001-0365-3 e-issn: 1432-072X issn: 0302-8933 |
Aparece en las colecciones: | (IMB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
35
checked on 23-abr-2024
WEB OF SCIENCETM
Citations
36
checked on 22-feb-2024
Page view(s)
341
checked on 23-abr-2024
Download(s)
104
checked on 23-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.