English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/66378
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Modelling the transmembrane arrangement of the uncoupling protein UCP1 and topological considerations of the nucleotide binding site

AuthorsLedesma, Amalia ; García de Lacoba, Mario ; Arechaga, Ignacio ; Rial, Eduardo
KeywordsUncoupling protein
UCP1
AAC
nucleotide binding
mitochondria
molecular modeling
Issue DateDec-2002
PublisherSpringer
CitationJournal of Bioenergetics and Biomembranes 34(6):473-486(2002)
AbstractThe uncoupling protein from brown adipose tissue (UCP1) is a mitochondrial proton transporter whose activity is inhibited by purine nucleotides. UCP1, like the other members of the mitochondrial transporter superfamily, is an homodimer and each subunit contains six transmembrane segments. In an attempt to understand the structural elements that are important for nucleotide binding, a model for the transmembrane arrangement of UCP1 has been built by computational methods. Biochemical and sequence analysis considerations are taken as constraints. The main features of the model include the following: (i) the six transmembrane α-helices (TMHs) associate to form an antiparallel helix bundle; (ii) TMHs have an amphiphilic nature and thus the hydrophobic and variable residues face the lipid bilayer; (iii) matrix loops do not penetrate in the core of the bundle; and (iv) the polar core constitutes the translocation pathway. Photoaffinity labeling and mutagenesis studies have identified several UCP1 regions that interact with the nucleotide. We present a model where the nucleotide binds deep inside the bundle core. The purine ring interacts with the matrix loops while the polyphosphate chain is stabilized through interactions with essential Arg residues in the TMH and whose side chains face the core of the helix bundle
Description14 páginas, 6 figuras -- PAGS nros. 473-486
Publisher version (URL)http://dx.doi.org/10.1023/A:1022522310279
URIhttp://hdl.handle.net/10261/66378
DOI10.1023/A:1022522310279
ISSN0145-479X
E-ISSN1573-6881
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
restringido.pdf21,67 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.