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Title

Disruption of the SHM2 gene, encoding one of two serine hydroxymethyltransferase isoenzymes, reduces the flux from glycine to serine in Ashbya gossypii

AuthorsSchlüpen, Christina; Santos García, María Ángeles; Revuelta Doval, José Luis
Issue Date2003
PublisherBiochemical Society
CitationBiochemical Journal 369: 263-273 (2003)
AbstractRiboflavin overproduction in the ascomycete Ashbya gossypii is limited by glycine, a precursor of purine biosynthesis, and therefore an indicator of glycine metabolism. Disruption of the SHM2 gene, encoding a serine hydroxymethyltransferase, resulted in a significant increase in riboflavin productivity. Determination of the enzyme's specific activity revealed a reduction from 3 m-units/mg of protein to 0.5 m-unit/mg protein. The remaining activity was due to an isoenzyme encoded by SHM1, which is probably mitochondrial. A hypothesis proposed to account for the enhanced riboflavin overproduction of SHM2-disrupted mutants was that the flux from glycine to serine was reduced, thus leading to an elevated supply with the riboflavin precursor glycine. Evidence for the correctness of that hypothesis was obtained from 13C-labelling experiments. When 500 οM 99% [1-13C]threonine was fed, more than 50% of the label was detected in C-1 of glycine resulting from threonine aldolase activity. More than 30% labelling determined in C-1 of serine can be explained by serine synthesis via serine hydroxymethyltransferase. Knockout of SHM1 had no detectable effect on serine labelling, but disruption of SHM2 led to a decrease in serine (2-5%) and an increase in glycine (59-67%) labelling, indicating a changed carbon flux.
URIhttp://hdl.handle.net/10261/66366
DOI10.1042/BJ20021224
Identifiersdoi: 10.1042/BJ20021224
issn: 0264-6021
e-issn: 1470-8728
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