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dc.contributor.authorGonzález-Barroso, M. Mar-
dc.contributor.authorLedesma, Amalia-
dc.contributor.authorLepper, Simone-
dc.contributor.authorPérez-Magán, Elisa-
dc.contributor.authorZaragoza, Pilar-
dc.contributor.authorRial, Eduardo-
dc.date.accessioned2013-02-07T12:36:28Z-
dc.date.available2013-02-07T12:36:28Z-
dc.date.issued2006-03-
dc.identifier.citationYeast, 23 (4) : 307-313 (2006)es_ES
dc.identifier.issn0749-503X-
dc.identifier.urihttp://hdl.handle.net/10261/66306-
dc.description7 páginas, 2 figuras, 1 tabla -- PAGS nros. 307-313es_ES
dc.description.abstractPichia pastoris is a methylotrophic yeast of high biotechnological interest. The bioenergetic properties of mitochondria from Pichia pastoris have not yet been determined. We report on a protocol for the isolation of the mitochondria in a state that shows good energy coupling. Analysis of Pichia pastoris growth and bioenergetic properties of the isolated mitochondria reveals that glycerol is the carbon source that yields the best results. Under our growth conditions, mitochondria oxidize external NADH but do not possess an alternative oxidase. Finally, Pichia pastoris mitochondria also lack the nucleotide-stimulated uncoupling pathway previously identified in Saccharomyces cerevisiaees_ES
dc.description.sponsorshipThis work is supported by a project grant from the Spanish Ministry Education and Science (BIO2002-00142). S.L. was supported by a grant from the Baden-Württemberg Stipendium programme (Germany)es_ES
dc.language.isoenges_ES
dc.publisherJohn Wiley & Sonses_ES
dc.rightsclosedAccesses_ES
dc.subjectMitochondriaes_ES
dc.subjectPichia pastorises_ES
dc.subjectyeastes_ES
dc.subjectmitochondrial respirationes_ES
dc.titleIsolation and bioenergetic characterization of mitochondria from Pichia pastorises_ES
dc.typeartículoes_ES
dc.identifier.doi10.1002/yea.1355-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1002/yea.1355es_ES
dc.identifier.e-issn1097-0061-
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