English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/66306
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Isolation and bioenergetic characterization of mitochondria from Pichia pastoris

AuthorsGonzález-Barroso, M. Mar ; Ledesma, Amalia ; Lepper, Simone; Pérez-Magán, Elisa; Zaragoza, Pilar ; Rial, Eduardo
Pichia pastoris
mitochondrial respiration
Issue DateMar-2006
PublisherJohn Wiley & Sons
CitationYeast, 23 (4) : 307-313 (2006)
AbstractPichia pastoris is a methylotrophic yeast of high biotechnological interest. The bioenergetic properties of mitochondria from Pichia pastoris have not yet been determined. We report on a protocol for the isolation of the mitochondria in a state that shows good energy coupling. Analysis of Pichia pastoris growth and bioenergetic properties of the isolated mitochondria reveals that glycerol is the carbon source that yields the best results. Under our growth conditions, mitochondria oxidize external NADH but do not possess an alternative oxidase. Finally, Pichia pastoris mitochondria also lack the nucleotide-stimulated uncoupling pathway previously identified in Saccharomyces cerevisiae
Description7 páginas, 2 figuras, 1 tabla -- PAGS nros. 307-313
Publisher version (URL)http://dx.doi.org/10.1002/yea.1355
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
restringido.pdf21,67 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.