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Título: | Yeast cultures with UCP1 uncoupling activity as a heating device |
Autor: | Delás, Joaquina; Notari, Meritxell; Forés, Jaume; Pechuan, Joaquín; Porcar, Manuel CSIC ORCID; Navarro, Emilio; Montagud, Arnau; Baguena, Minerva; Peretó, Juli CSIC ORCID; Córdoba, Pedro; González-Barroso, M. Mar; Rial, Eduardo CSIC ORCID ; Moya-Simarro, Andrés; Urchueguía, Javier | Palabras clave: | Calorimetry Instrumentation ion Channels Metabolism mitochondrial Proteins Saccharomyces cerevisiae Cytology Culture heating devices |
Fecha de publicación: | 31-dic-2009 | Editor: | Elsevier | Citación: | New Biotechnology 26(6):300-306(2009) | Resumen: | Uncoupling proteins (UCPs) are mitochondrial transporters that facilitate controlled dissipation of the proton gradient and thus regulate energetic efficiency. The heat generating capacity of UCP from brown adipose tissue was investigated in yeasts expressing the protein recombinantly under conditions in which the temperature of the growth medium was measured directly. A Liquid Culture Calorimeter (LCC) was built consisting of a thermally isolated culture flask able to keep yeast cultures warm without resorting to additional heating. The exact internal temperature of the cultures was monitored for 24 h through a thermocouple connected to a data logger. Under these conditions, significant temperature increases (1 °C) in the media were recorded when yeast strains expressing endogenously active UCP1 mutants were grown. This is the first direct evidence, in a eukaryotic microbial model, of a temperature rise associated with uncoupling activity, and could be seen as the first step toward developing a biological heating device | Descripción: | 7 páginas, 5 figuras, 3 tablas -- PAGS nros. 300-306 | Versión del editor: | http://dx.doi.org/10.1016/j.nbt.2009.09.005 | URI: | http://hdl.handle.net/10261/66180 | DOI: | 10.1016/j.nbt.2009.09.005 | ISSN: | 1871-6784 |
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