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dc.contributor.authorDelás, Joaquina-
dc.contributor.authorNotari, Meritxell-
dc.contributor.authorForés, Jaume-
dc.contributor.authorPechuan, Joaquín-
dc.contributor.authorPorcar, Manuel-
dc.contributor.authorNavarro, Emilio-
dc.contributor.authorMontagud, Arnau-
dc.contributor.authorBaguena, Minerva-
dc.contributor.authorPeretó, Juli-
dc.contributor.authorCórdoba, Pedro-
dc.contributor.authorGonzález-Barroso, M. Mar-
dc.contributor.authorRial, Eduardo-
dc.contributor.authorMoya-Simarro, Andrés-
dc.contributor.authorUrchueguía, Javier-
dc.date.accessioned2013-02-06T12:49:56Z-
dc.date.available2013-02-06T12:49:56Z-
dc.date.issued2009-12-31-
dc.identifier.citationNew Biotechnology 26(6):300-306(2009)es_ES
dc.identifier.issn1871-6784-
dc.identifier.urihttp://hdl.handle.net/10261/66180-
dc.description7 páginas, 5 figuras, 3 tablas -- PAGS nros. 300-306es_ES
dc.description.abstractUncoupling proteins (UCPs) are mitochondrial transporters that facilitate controlled dissipation of the proton gradient and thus regulate energetic efficiency. The heat generating capacity of UCP from brown adipose tissue was investigated in yeasts expressing the protein recombinantly under conditions in which the temperature of the growth medium was measured directly. A Liquid Culture Calorimeter (LCC) was built consisting of a thermally isolated culture flask able to keep yeast cultures warm without resorting to additional heating. The exact internal temperature of the cultures was monitored for 24 h through a thermocouple connected to a data logger. Under these conditions, significant temperature increases (1 °C) in the media were recorded when yeast strains expressing endogenously active UCP1 mutants were grown. This is the first direct evidence, in a eukaryotic microbial model, of a temperature rise associated with uncoupling activity, and could be seen as the first step toward developing a biological heating devicees_ES
dc.description.sponsorshipThis work was presented as ‘The Hot Yeast Project’ by the Valencia team in the 2008 iGEM competition. The team was supported by Vicerrectorado de Investigación, Desarrollo e Innovación (Universidad Politécnica de Valencia), Ingenio Mathematica i-math Project and European community's Seventh Framework Programme (FP7/2007-2013) under grant agreement no 212894 ‘Targeting environmental pollution with engineered microbial systems à la carte’ (TARPOL). Research was financially supported by Vicerrectorat d’Investigació (Universitat de València). Manuel Porcar has a ‘Ramon y Cajal’ research contract and Emilio Navarro a ‘Juan de la Cierva’ research contract both from the Spanish Ministerio de Ciencia e Innovación. Eduardo Rial is supported by research grants from the Ministerio de Ciencia e Innovación (BFU2006-08182 and Consolider-Ingenio 2010 CSD2007-00020es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsclosedAccesses_ES
dc.subjectCalorimetryes_ES
dc.subjectinstrumentationes_ES
dc.subjection Channelses_ES
dc.subjectmetabolismes_ES
dc.subjectmitochondrial Proteinses_ES
dc.subjectSaccharomyces cerevisiaees_ES
dc.subjectcytologyes_ES
dc.subjectculturees_ES
dc.subjectheatinges_ES
dc.subjectdeviceses_ES
dc.titleYeast cultures with UCP1 uncoupling activity as a heating devicees_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.nbt.2009.09.005-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.nbt.2009.09.005es_ES
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